Human IL-13Ra2 Protein (ILR-HM1R2)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 8.6ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asp27-Leu342

Catalog: ILR-HM1R2

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Product Description	Recombinant Human IL-13Ra2 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Asp27-Leu342.
Product Background	Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.
Product Category	Recombinant Protein

Target	IL-13Ra2
Accession	Q14627
Species	Human
Amino Acid Range	Asp27-Leu342
Molecular Weight	The protein has a predicted MW of 38.28 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 8.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human IL-13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 8.6ng/ml determined by ELISA.
	The purity of Human IL-13Ra2 is greater than 95% as determined by SEC-HPLC.
	Human IL-13Ra2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.