Human IL-15 Protein (IL5-HE015)

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-15, No Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4 ng/ml determined by ELISA. The affinity constant of 5.9 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asn49-Ser162

Catalog: IL5-HE015

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Product Description	Recombinant Human IL-15 Protein is expressed from E.coli without tag.It contains Asn49-Ser162.
Product Background	Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
Product Category	Recombinant Protein

Target	IL-15
Synonyms	L15; IL-15; IL-15MGC9721; Interleukin-15
Accession	P40933
Species	Human
Amino Acid Range	Asn49-Ser162
Molecular Weight	The protein has a predicted MW of 12.8 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Human IL-15, No Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4 ng/ml determined by ELISA. The affinity constant of 5.9 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.05 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4) or 20 mM Tris, 150 mM NACI (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-15, No Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4 ng/ml determined by ELISA.
	Measured in a cell proliferation assay using CTLL-2 cells. The ED50-for this effect is 0.1 - 0.3 ng/mL. The specific activity of recombinant human IL-15 is > 1 x 10^7-units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554) (QC Test).
	The purity of Human IL-15 is greater than 95% as determined by SEC-HPLC.
	Human IL-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.