Product Description |
Recombinant Human IL-15 Protein is expressed from E.coli without tag.It contains Asn49-Ser162. |
Product Background |
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA. |
Product Category |
Recombinant Protein |
Target |
IL-15 |
Synonyms |
L15; IL-15; IL-15MGC9721; Interleukin-15 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asn49-Ser162 |
Molecular Weight |
The protein has a predicted MW of 12.8 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-15, No Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4 ng/ml determined by ELISA. The affinity constant of 5.9 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.05 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20 mM Tris, 150 mM NACI (pH 7.5). Normally 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human IL-15, No Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4 ng/ml determined by ELISA. |
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Human IL-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Measured in a cell proliferation assay using CTLL-2 cells. The ED50-for this effect is 0.1 - 0.3 ng/mL. The specific activity of recombinant human IL-15 is > 1 × 10^7-units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554) (QC Test). |
|
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The purity of Human IL-15 is greater than 95% as determined by SEC-HPLC. |
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