Name: Human IL-15 Protein (IL5-HE015)
Brand: KactusBio
SKU: IL5-HE015-100UG
Price: 280.5 USD
Availability: InStock

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-15, No Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4 ng/ml determined by ELISA. The affinity constant of 5.9 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asn49-Ser162

Catalog #IL5-HE015

Size

100UG

500UG

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Human IL-15 Protein (IL5-HE015) Summary

Product Description	Recombinant Human IL-15 Protein is expressed from E.coli without tag.It contains Asn49-Ser162.
Product Background	Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human IL-15 Protein (IL5-HE015) Specifications/Details

Protein	IL-15
Synonyms	L15; IL-15; IL-15MGC9721; Interleukin-15
Accession	P40933
Species	Human
Biotinylated	no
Amino Acid Range	Asn49-Ser162
Molecular Weight	The protein has a predicted MW of 12.8 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human IL-15, No Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4 ng/ml determined by ELISA. The affinity constant of 5.9 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.05 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20 mM Tris, 150 mM NACI (pH 7.5). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-15, No Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 15.4 ng/ml determined by ELISA.
	Human IL-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using CTLL-2 cells. The ED50-for this effect is 0.1 - 0.3 ng/mL. The specific activity of recombinant human IL-15 is > 1 × 10^7-units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554) (QC Test).
	The purity of Human IL-15 is greater than 95% as determined by SEC-HPLC.