Human IL-15RA/IL-15 R alpha/CD215 Protein (ILR-HM215)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-15, No Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, hFc Tag with the EC50 of 30.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ile31-Thr205

Catalog: ILR-HM215

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Product Description Recombinant Human IL-15RA/IL-15 R alpha/CD215 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile31-Thr205.

Product Background

Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.

Product Category

Recombinant Protein

Target

IL-15RA/IL-15 R alpha/CD215
Synonyms IL-15 R alpha; CD215; IL-15RA; MGC104179

Gene/Protein ID

Q13261-1

Species

Human

Exact Sequence

Ile31-Thr205
Molecular Weight The protein has a predicted MW of 45.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-15, No Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, hFc Tag with the EC50 of 30.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-15, No Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, hFc Tag with the EC50 of 30.2ng/ml determined by ELISA.
The purity of Human IL-15RA is greater than 95% as determined by SEC-HPLC.
Human IL-15RA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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