• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-15, No Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, hFc Tag with the EC50 of 24.0ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ile31-Thr205
Catalog #ILR-HM215-UL
Price
Regular price $32670
Regular price Sale price $32670
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Size
Avantor VWR Fisher Scientific

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Human IL-15RA/IL-15 R alpha/CD215 Protein, Ultra Low Endotoxin (ILR-HM215-UL) Summary

Product Description

 Recombinant Human IL-15RA/IL-15 R alpha/CD215 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ile31-Thr205.

Product Background

 Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.

Human IL-15RA/IL-15 R alpha/CD215 Protein, Ultra Low Endotoxin (ILR-HM215-UL) Specifications/Details

Protein
 IL-15RA/IL-15 R alpha/CD215
Synonyms
 IL-15 R alpha; CD215; IL-15RA; MGC104179;IL15R alpha; IL15RA
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ile31-Thr205
Molecular Weight
 The protein has a predicted MW of 45.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-15, No Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, hFc Tag with the EC50 of 24.0ng/ml determined by ELISA (QC Test).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-15, No Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, hFc Tag with the EC50 of 24.0ng/ml determined by ELISA (QC Test). Immobilized Human IL-15, No Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, hFc Tag with the EC50 of 24.0ng/ml determined by ELISA (QC Test).
Human IL-15RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-15RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-15RA is greater than 95% as determined by SEC-HPLC. The purity of Human IL-15RA is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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