Human IL-17A/CTLA-8 Protein (ILA-HM118)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-17A, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 0.36μg/ml determined by ELISA (QC Test). The affinity constant of 1.57nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly24-Ala155

Catalog: ILA-HM118

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Product Description Recombinant Human IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag at the C-terminal.It contains Gly24-Ala155.
Product Category Recombinant Protein
Target IL-17A/CTLA-8
Synonyms CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A
Gene/Protein ID Q16552-1
Species Human
Exact Sequence Gly24-Ala155
Molecular Weight The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 16-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-17A, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 0.36μg/ml determined by ELISA (QC Test). The affinity constant of 1.57nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-17R alpha, His Tag immobilized on CM5 Chip can bind Human IL-17A, His Tag with an affinity constant of 1.57nM as determined in SPR assay (Biacore T200) (Routine Test).
Immobilized Human IL-17A, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 0.36μg/ml determined by ELISA (QC Test).
Immobilized Human IL-17A, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 60.6ng/ml determined by ELISA (Routine Test).
Immobilized Human IL-17A, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 66.6ng/ml determined by ELISA (Routine Test).
The purity of Human IL-17A is greater than 95% as determined by SEC-HPLC.
Human IL-17A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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