Human IL-17A/CTLA-8 Protein (ILA-HM118)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-17A, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA. The affinity constant of 1.57nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly24-Ala155
Catalog #ILA-HM118
/
Size

Order now, ships by:

Product Description
 Recombinant Human IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Ala155.
Product Background
 Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Product Category
 Recombinant Protein
Target
 IL-17A/CTLA-8
Synonyms
 CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly24-Ala155
Molecular Weight
 The protein has a predicted MW of 16.2 kDa. Due to glycosylation, the protein migrates to 15-20 kDa and 25-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-17A, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA. The affinity constant of 1.57nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-17R alpha, His Tag immobilized on CM5 Chip can bind Human IL-17A, His Tag with an affinity constant of 1.57nM as determined in SPR assay (Biacore T200) (Routine Test).
Immobilized Human IL-17A, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA.
Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test).
Immobilized Human IL-17A, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
Human IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-17A is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed