Human IL-17A/CTLA-8 Protein (ILA-HM118)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-17A, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA (Routine Test). The affinity constant of 1.57nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly24-Ala155

Catalog: ILA-HM118

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Product Description Recombinant Human IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Ala155.

Product Background

Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.

Product Category

Recombinant Protein

Target

IL-17A/CTLA-8
Synonyms CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A
Accession

Species

Human

Amino Acid Range

Gly24-Ala155
Molecular Weight The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 16-30 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-17A, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA (Routine Test). The affinity constant of 1.57nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-17R alpha, His Tag immobilized on CM5 Chip can bind Human IL-17A, His Tag with an affinity constant of 1.57nM as determined in SPR assay (Biacore T200) (Routine Test).
Immobilized Human IL-17A, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA (Routine Test).
Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (Routine Test).
Immobilized Human IL-17A, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).
The purity of Human IL-17A is greater than 95% as determined by SEC-HPLC.
Human IL-17A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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