Name: Human IL-17A/CTLA-8 Protein (ILA-HM118)
Brand: KactusBio
SKU: ILA-HM118-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test). The affinity constant of 1.57nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly24-Ala155

Catalog #ILA-HM118

Size

100UG

500UG

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Product Description	Recombinant Human IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Ala155.
Product Background	Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Product Category	Recombinant Protein / Antibody discovery

Protein	IL-17A/CTLA-8
Synonyms	CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A
Accession	Q16552-1
Species	Human
Biotinylated	no
Amino Acid Range	Gly24-Ala155
Molecular Weight	The protein has a predicted MW of 16.2 kDa. Due to glycosylation, the protein migrates to 15-20 kDa and 25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test). The affinity constant of 1.57nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-17R alpha, His Tag immobilized on CM5 Chip can bind Human IL-17A, His Tag with an affinity constant of 1.57nM as determined in SPR assay (Biacore T200) (Routine Test).
	Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test).
	Immobilized Human IL-17A, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
	Immobilized Human IL-17A, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 33.8ng/ml determined by ELISA.
	Human IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IL-17A is greater than 95% as determined by SEC-HPLC.