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Human IL-17A&F Protein (IAF-HM117) Summary
Product Description |
Recombinant Human IL-17A&F Protein is expressed as a heterodimer of IL-17A and IL-17F by co-expression in HEK293. Subunit IL-17A is fused with a Strep II tag at the N-terminus and subunit IL-17F is fused with a His tag at the N-terminus.It contains Gly24-Ala155 (IL-17A) and Arg31-Gln163 (IL-17F). |
Product Background |
Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human IL-17A&F Protein (IAF-HM117) Specifications/Details
Protein |
IL-17A&F |
Synonyms |
IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,IL24,ML-1,Interleukin-17F |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F) |
Molecular Weight |
The protein has a predicted MW of 16.16 kDa (IL-17A) and 16 kDa (IL-17F). Due to glycosylation, the protein migrates to 17-23 kDa and 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 62.6ng/ml determined by ELISA. |
Product Tag |
N-Strep-II, N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag (Cat. CD7-HM2RA) with the EC50 of 62.6ng/ml determined by ELISA. |
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Immobilized Human IL-17A&F, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag (Cat. IL1-HM2RC) with the EC50 of 85.2ng/ml determined by ELISA. |
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Immobilized Human IL-17A&F, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 1.3ng/ml determined by ELISA. |
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Human IL-17A&F on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human IL-17A&F is greater than 95% and the molecular weight of this protein is around 37-50 kDa as determined by SEC-MALS. |
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The purity of Human IL-17A&F is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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