Human IL-17A&F Protein (ILF-HM118)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)

Catalog: ILF-HM118

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Product Description Recombinant Human IL-17A&F Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gly24-Ala155(IL-17A)&Arg31-Gln163(IL-17F).
Product Category Recombinant Protein
Target IL-17A&F
Synonyms IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,IL24,ML-1,Interleukin-17F
Gene/Protein ID Q16552-1(IL-17A)&Q96PD4(IL-17F)
Species Human
Exact Sequence Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)
Molecular Weight The protein has a predicted MW of 15.1 kDa (IL-17A) & 15.7 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa & 27 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-17A&F, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA.
The purity of Human IL-17A&F is greater than 95% as determined by SEC-HPLC.
Human IL-17A&F on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%

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