Product Description |
Recombinant Human IL-17A&F Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Ala155(IL-17A)&Arg31-Gln163(IL-17F). |
Product Background |
Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines. |
Product Category |
Recombinant Protein |
Target |
IL-17A&F |
Synonyms |
IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,IL24,ML-1,Interleukin-17F |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F) |
Molecular Weight |
The protein has a predicted MW of 15.1 kDa (IL-17A) & 15.7 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa & 27 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-17A&F, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human IL-17A&F, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA. |
|
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Human IL-17A&F on Bis-Tris PAGE under reduced conditions. The purity is greater than 95% |
|
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The purity of Human IL-17A&F is greater than 95% as determined by SEC-HPLC. |
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