Human IL-17A&F Protein (ILF-HM118)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-17A&F, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 8.9ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)

Catalog: ILF-HM118

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Product Description	Recombinant Human IL-17A&F Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Ala155(IL-17A)&Arg31-Gln163(IL-17F).
Product Background	Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Product Category	Recombinant Protein

Target	IL-17A&F
Synonyms	IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,IL24,ML-1,Interleukin-17F
Gene/Protein ID	Q16552-1(IL-17A)&Q96PD4(IL-17F)
Species	Human
Exact Sequence	Gly24-Ala155(IL-17A) & Arg31-Gln163(IL-17F)
Molecular Weight	The protein has a predicted MW of 15.1 kDa (IL-17A) & 15.7 kDa (IL-17F). Due to glycosylation, the protein migrates to 16 kDa & 27 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-17A&F, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 8.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human IL-17A&F, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 8.9ng/ml determined by ELISA.
	The purity of Human IL-17A&F is greater than 95% as determined by SEC-HPLC.
	Human IL-17A&F on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%