Product Description |
Recombinant Human IL-17C Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His19-Val197. |
Product Background |
Interleukin‑17C (IL‑17C) is a 15‑20 kDa glycosylated cytokine that plays an important role in mucosal immunity and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold. IL‑17C is Cytokine that plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. Stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NF-kappa-B and MAPK pathways. Acts synergically with IL22 in inducing the expression of antibacterial peptides, including S100A8, S100A9, REG3A and REG3G. |
Product Category |
Recombinant Protein |
Target |
IL-17C |
Synonyms |
CX2; Cytokine CX2; IL17C; IL-17C; IL-17CMGC126884 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
His19-Val197 |
Molecular Weight |
The protein has a predicted MW of 22.6 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-17C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17RE, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test). The affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by SEC-HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM MES, 150mM NaCl (PH 6.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (PH 6.0). |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human IL-17R alpha, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-17C, His Tag-with an affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human IL-17C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17RE, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test). |
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|
Human IL-17C on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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