Human IL-17R alpha/CD217 Protein (CD7-HM1RA)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-17R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17A, His Tag with the EC50 of 0.44ug/ml determined by ELISA (QC Test). The affinity constant of 1.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu33-Trp320
Catalog #CD7-HM1RA
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Product Description
 Recombinant Human IL-17R alpha/CD217 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu33-Trp320.
Product Background
 Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22.
Product Category
 Recombinant Protein
Target
 IL-17R alpha/CD217
Synonyms
 CD217; CDw217; IL-17RA; IL17R; CANDF5; IL-7R; IL-7R-alpha; ILRA
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu33-Trp320
Molecular Weight
 The protein has a predicted MW of 34.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-17R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17A, His Tag with the EC50 of 0.44ug/ml determined by ELISA (QC Test). The affinity constant of 1.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-17R alpha, His Tag immobilized on CM5 Chip can bind Human IL-17A, His Tag with an affinity constant of 1.57 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-17R alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17A, His Tag with the EC50 of 0.44ug/ml determined by ELISA (QC Test).
Human IL-17R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-17R alpha is greater than 95% as determined by SEC-HPLC.

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