Human IL-17R alpha/CD217 Protein (CD7-HM1RA)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-17R alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17A, His Tag with the EC50 of 0.15ug/ml determined by ELISA (QC Test). The affinity constant of 1.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Leu33-Trp320

Catalog: CD7-HM1RA

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Product Description	Recombinant Human IL-17R alpha/CD217 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu33-Trp320.
Product Background	Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22.
Product Category	Recombinant Protein

Target	IL-17R alpha/CD217
Synonyms	CD217; CDw217; IL-17RA; IL17R; CANDF5; IL-7R; IL-7R-alpha; ILRA
Gene/Protein ID	Q96F46-1
Species	Human
Exact Sequence	Leu33-Trp320
Molecular Weight	The protein has a predicted MW of 34.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-17R alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17A, His Tag with the EC50 of 0.15ug/ml determined by ELISA (QC Test). The affinity constant of 1.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-17R alpha, His Tag immobilized on CM5 Chip can bind Human IL-17A, His Tag with an affinity constant of 1.57 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-17R alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-17A, His Tag with the EC50 of 0.15ug/ml determined by ELISA (QC Test).
	The purity of Human IL-17R alpha is greater than 95% as determined by SEC-HPLC.
	Human IL-17R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.