Human IL-17R alpha/CD217 Protein (CD7-HM2RA)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 72.2ng/ml determined by ELISA. The affinity constant of 2.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu33-Trp320
Catalog #CD7-HM2RA
Regular price $148.50
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Product Description

Recombinant Human IL-17R alpha/CD217 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu33-Trp320.

Product Background

Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

IL-17R alpha/CD217

Synonyms

CD217; CDw217; IL-17RA; IL17R; CANDF5; IL-17R; IL-17R-alpha; IL17Rα

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Leu33-Trp320

Molecular Weight

The protein has a predicted MW of 60.2 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 72.2ng/ml determined by ELISA. The affinity constant of 2.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IL-17R alpha, hFc Tag captured on CM5 Chip via protein A can bind Biotinylated Human IL-17A, His-Avi Tag with an affinity constant of 2.18 nM as determined in SPR assay (Biacore T200). Human IL-17R alpha, hFc Tag captured on CM5 Chip via protein A can bind Biotinylated Human IL-17A, His-Avi Tag with an affinity constant of 2.18 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 72.2ng/ml determined by ELISA. Immobilized Human IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 72.2ng/ml determined by ELISA.
Immobilized Human IL-17F, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17 R alpha, hFc Tag with the EC50 of 1.75ug/ml determined by ELISA. Immobilized Human IL-17F, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17 R alpha, hFc Tag with the EC50 of 1.75ug/ml determined by ELISA.
Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 44.1ng/ml determined by ELISA. Immobilized Human IL-17A&F, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 44.1ng/ml determined by ELISA.
Human IL-17R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-17R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-17R alpha is greater than 95% as determined by SEC-HPLC. The purity of Human IL-17R alpha is greater than 95% as determined by SEC-HPLC.

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