Human IL-17R alpha/CD217 Protein (CD7-HM2RA)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-17A, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 78.0ng/ml determined by ELISA. The affinity constant of 2.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Leu33-Trp320

Catalog: CD7-HM2RA

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Product Description Recombinant Human IL-17R alpha/CD217 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Leu33-Trp320.
Product Category Recombinant Protein
Target IL-17R alpha/CD217
Synonyms CD217; CDw217; IL-17RA; IL17R; CANDF5; IL-7R; IL-7R-alpha; ILRA
Gene/Protein ID Q96F46-1
Species Human
Exact Sequence Leu33-Trp320
Molecular Weight The protein has a predicted MW of 60.2 kDa. Due to glycosylation, the protein migrates to 75-90 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-17A, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 78.0ng/ml determined by ELISA. The affinity constant of 2.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-17R alpha, hFc Tag captured on CM5 Chip via protein A can bind Biotinylated Human IL-17A, His-Avi Tag with an affinity constant of 2.18 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-17A, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 78.0ng/ml determined by ELISA.
The purity of Human IL-17R alpha is greater than 95% as determined by SEC-HPLC.
Human IL-17R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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