Human IL-17RB Protein (IL1-HM1RB)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-17RB, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17RB Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.81 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Arg18-Per292
Catalog #IL1-HM1RB
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Product Description
 Recombinant Human IL-17RB Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg18-Per292.
Product Background
 Among inflammatory mediators, a growing body of evidence emphasizes the contribution of the interleukin 17 (IL-17) cytokine family in malignant diseases. Besides IL-17A, the prototypic member of the IL-17 family, several experimental findings strongly support the role of the IL-17B/IL-17 receptor B (IL-17RB) pathway in tumorigenesis and resistance to anticancer therapies. In mouse models, IL-17B signaling through IL-17RB directly promotes cancer cell survival, proliferation, and migration, and induces resistance to conventional chemotherapeutic agents.
Product Category
 Recombinant Protein
Target
 IL-17RB
Synonyms
 IL-17 receptor B; IL-17RB; IL-17ER; Evi27; IL-17 RH1; IL-17ER; IL17RB
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Arg18-Per292
Molecular Weight
 The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-17RB, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17RB Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.81 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-17RB, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17RB Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).
Immobilized Human IL-17RB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA.
Human IL-17RB on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-17RB is greater than 95% as determined by SEC-HPLC.
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