Name: Human IL-18 Protein (IL1-HE018)
Brand: KactusBio
SKU: IL1-HE018-100UG
Price: 330.0 USD
Availability: InStock

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 62.0ng/ml determined by ELISA (QC Test). The affinity constant of 2.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Tyr37-Asp193

Catalog #IL1-HE018

Size

100UG

500UG

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Human IL-18 Protein (IL1-HE018) Summary

Product Description	Recombinant Human IL-18 Protein is expressed from E.coli without tag.It contains Tyr37-Asp193.
Product Background	Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human IL-18 Protein (IL1-HE018) Specifications/Details

Protein	IL-18
Synonyms	IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1; IL-1γ; IL-1 γ
Accession	Q14116
Species	Human
Biotinylated	no
Amino Acid Range	Tyr37-Asp193
Molecular Weight	The protein has a predicted MW of 18.2 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 62.0ng/ml determined by ELISA (QC Test). The affinity constant of 2.38 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM PB, 250mM NaCl, 50mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 62.0ng/ml determined by ELISA (QC Test).
	Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50-for this effect is 1-5 ng/mL (QC Test).
	Loaded Rhesus macaque IL-18 R1, hFc Tag on ProA-Biosensor, can bind Human IL-18, No Tag with an affinity constant of 2.01 nM as determined in BLI assay (Gator).
	The purity of Human IL-18 is greater than 95% as determined by SEC-HPLC.