Human IL-18 Protein (IL1-HE018)
Human IL-18 Protein (IL1-HE018)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 62.0ng/ml determined by ELISA (QC Test). The affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Tyr37-Asp193
Catalog #IL1-HE018
Regular price $330.00
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Human IL-18 Protein (IL1-HE018) Summary

Product Description

Recombinant Human IL-18 Protein is expressed from E.coli without tag.It contains Tyr37-Asp193.

Product Background

Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human IL-18 Protein (IL1-HE018) Specifications/Details

Protein
IL-18
Synonyms
IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1; IL-1γ; IL-1 γ
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Tyr37-Asp193
Molecular Weight
The protein has a predicted MW of 18.2 kDa same as Bis-Tris PAGE result.
Activity
Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 62.0ng/ml determined by ELISA (QC Test). The affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).
Product Tag
No Tag
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 20mM PB, 250mM NaCl, 50mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200). Human IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 62.0ng/ml determined by ELISA (QC Test). Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 62.0ng/ml determined by ELISA (QC Test).
Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50-for this effect is 1-5 ng/mL (QC Test). Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50-for this effect is 1-5 ng/mL (QC Test).
Loaded Rhesus macaque IL-18 R1, hFc Tag on ProA-Biosensor, can bind Human IL-18, No Tag with an affinity constant of 2.01 nM as determined in BLI assay (Gator). Loaded Rhesus macaque IL-18 R1, hFc Tag on ProA-Biosensor, can bind Human IL-18, No Tag with an affinity constant of 2.01 nM as determined in BLI assay (Gator).
The purity of Human IL-18 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-18 is greater than 95% as determined by SEC-HPLC.
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