Product Description |
Recombinant Human IL-18 Protein is expressed from E.coli without tag.It contains Tyr37-Asp193. |
Product Background |
Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation. |
Product Category |
Recombinant Protein |
Target |
IL-18 |
Synonyms |
IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Tyr37-Asp193 |
Molecular Weight |
The protein has a predicted MW of 18.2 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). The affinity constant of 2.38 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM PB, 250mM NaCl, 50mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.38 nM as determined in SPR assay (Biacore T200). |
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|
Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). |
|
|
Human IL-18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50-for this effect is 1-5 ng/mL (QC Test). |
|
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The purity of Human IL-18 is greater than 95% as determined by SEC-HPLC. |
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