Human IL-18BP Protein (IL8-HM1BP)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: The affinity constant of 0.26nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Thr31-Gly194

Catalog: IL8-HM1BP

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Product Description Recombinant Human IL-18BP Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Thr31-Gly194.
Product Category Recombinant Protein
Target IL-18BP
Synonyms IL-18BP; Igifbp
Gene/Protein ID O95998-2
Species Human
Exact Sequence Thr31-Gly194
Molecular Weight The protein has a predicted MW of 18.7 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.26nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-18BP, His Tag captured on CM5 Chip via anti-His antibody can bind Human IL-18, No Tag with an affinity constant of 0.26nM as determined in SPR assay (Biacore T200).
The purity of Human IL-18BP is greater than 95% as determined by SEC-HPLC.
Human IL-18BP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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