Human IL-18BP Protein (IL8-HM1BP)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: The affinity constant of 0.41 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Thr31-Gly194

Catalog: IL8-HM1BP

$120.00
Size:

Order now get it on

Product Description Recombinant Human IL-18BP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr31-Gly194.

Product Background

Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.

Product Category

Recombinant Protein

Target

IL-18BP
Synonyms IL-18BP; Igifbp
Accession

Species

Human

Amino Acid Range

Thr31-Gly194
Molecular Weight The protein has a predicted MW of 18.7 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 0.41 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-18BP, His Tag captured on CM5 Chip via Anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.41 nM as determined in SPR assay (Biacore T200).
Measured by its ability to inhibit the IL-18-induced response of human natural killer lymphoma NK-92 cells. The ED50-for this effect is 0.04 - 0.08 µg/mL in the presence of 30 ng/mL of recombinant Human IL-18 (QC Test).
Human IL-18BP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in