Name: Human IL-18BP Protein (IL8-HM1BP)
Brand: KactusBio
SKU: IL8-HM1BP-100UG
Price: 132.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: The affinity constant of 0.41 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Thr31-Gly194

Catalog #IL8-HM1BP

Size

100UG

500UG

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Product Description	Recombinant Human IL-18BP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr31-Gly194.
Product Background	Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.
Product Category	Recombinant Protein / Antibody discovery

Protein	IL-18BP
Synonyms	IL-18BP; Igifbp
Accession	O95998-2
Species	Human
Biotinylated	no
Amino Acid Range	Thr31-Gly194
Molecular Weight	The protein has a predicted MW of 18.7 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.41 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-18BP, His Tag captured on CM5 Chip via Anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.41 nM as determined in SPR assay (Biacore T200).
	Human IL-18BP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to inhibit the IL-18-induced response of human natural killer lymphoma NK-92 cells. The ED50-for this effect is 0.04 - 0.08 µg/mL in the presence of 30 ng/mL of recombinant Human IL-18 (QC Test).