Product Description |
Recombinant Human IL-18BP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr31-Gly194. |
Product Background |
Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice. |
Product Category |
Recombinant Protein |
Target |
IL-18BP |
Synonyms |
IL-18BP; Igifbp |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr31-Gly194 |
Molecular Weight |
The protein has a predicted MW of 18.7 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.41 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human IL-18BP, His Tag captured on CM5 Chip via Anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.41 nM as determined in SPR assay (Biacore T200). |
|
|
Human IL-18BP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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|
Measured by its ability to inhibit the IL-18-induced response of human natural killer lymphoma NK-92 cells. The ED50-for this effect is 0.04 - 0.08 µg/mL in the presence of 30 ng/mL of recombinant Human IL-18 (QC Test). |
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