Human IL-18BP Protein (IL8-HM1BP)
Human IL-18BP Protein (IL8-HM1BP)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: The affinity constant of 0.41 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Thr31-Gly194
Catalog #IL8-HM1BP
Regular price $120.00
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Product Description
Recombinant Human IL-18BP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr31-Gly194.
Product Background
Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.
Product Category
Recombinant Protein
Target
IL-18BP
Synonyms
IL-18BP; Igifbp
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Thr31-Gly194
Molecular Weight
The protein has a predicted MW of 18.7 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 0.41 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-18BP, His Tag captured on CM5 Chip via Anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.41 nM as determined in SPR assay (Biacore T200).
Human IL-18BP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to inhibit the IL-18-induced response of human natural killer lymphoma NK-92 cells. The ED50-for this effect is 0.04 - 0.08 µg/mL in the presence of 30 ng/mL of recombinant Human IL-18 (QC Test).

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