Human IL-18BP Protein (IL8-HM2BP)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.04nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Thr31-Gly194

Catalog: IL8-HM2BP

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Product Description	Recombinant Human IL-18BP Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Thr31-Gly194.
Product Background	Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.
Product Category	Recombinant Protein

Target	IL-18BP
Synonyms	IL-18BP; Igifbp
Gene/Protein ID	O95998-2
Species	Human
Exact Sequence	Thr31-Gly194
Molecular Weight	The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.04nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-18BP, hFc Tag captured on Protein A chip, can bind Human IL-18 with an affinity constant of 0.04nM as determined in a SPR assay (Biacore T200).
	Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test).
	Human IL-18BP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.