Name: Human IL-1R1 Protein (IL1-HM1R1)
Brand: KactusBio
SKU: IL1-HM1R1-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-1R1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 58.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Leu18-Thr332
Other Available Proteins:

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Catalog #IL1-HM1R1

Size

100UG

500UG

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Human IL-1R1 Protein (IL1-HM1R1) Summary

Product Description	Recombinant Human IL-1R1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu18-Thr332.
Product Background	Interleukin 1 (IL-1) has long been known for its pleiotropic effects on inflammation that plays a complex, and sometimes contrasting, role in different stages of cancer development.IL-1R1 is the main receptor for both ligands and is expressed by various cell types, including innate and adaptive immune cell types, epithelial cells, endothelial cells, adipocytes, chondrocytes, fibroblasts, etc. IL-1 and IL-1R1 receptor interaction leads to a set of common signaling pathways, mainly the NF-kB and MAP kinase pathways, as a result of complex positive and negative regulations.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human IL-1R1 Protein (IL1-HM1R1) Specifications/Details

Protein	IL-1R1
Synonyms	IL-1R-1; IL-1RT1; IL-1R-alpha; CD121a; IL-1 RI; IL1R; IL1RI; IL-1RI; p80; D2S1473; IL1RA
Accession	NP_000868.1
Species	Human
Biotinylated	no
Amino Acid Range	Leu18-Thr332
Molecular Weight	The protein has a predicted MW of 37 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-1R1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 58.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-1R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-1 alpha, No tag with an affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).
	Human IL-1R1, His Tag immobilized on CM5 Chip can bind Biotinylated Human IL-1 alpha, His Tag with an affinity constant of 0.76 uM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-1R1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 58.8ng/ml determined by ELISA (QC Test).
	Human IL-1R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human IL-1R1, His Tag on Anti-His-Biosensor can bind Human IL-1 alpha, No Tag (Cat. IL1-HE00A) with an affinity constant of 0.84 nM as determined in BLI assay .
	The purity of Human IL-1R1 is greater than 95% as determined by SEC-HPLC.

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