Product Description |
Recombinant Human IL-1R1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu18-Thr332. |
Product Background |
Interleukin 1 (IL-1) has long been known for its pleiotropic effects on inflammation that plays a complex, and sometimes contrasting, role in different stages of cancer development.IL-1R1 is the main receptor for both ligands and is expressed by various cell types, including innate and adaptive immune cell types, epithelial cells, endothelial cells, adipocytes, chondrocytes, fibroblasts, etc. IL-1 and IL-1R1 receptor interaction leads to a set of common signaling pathways, mainly the NF-kB and MAP kinase pathways, as a result of complex positive and negative regulations. |
Product Category |
Recombinant Protein |
Target |
IL-1R1 |
Synonyms |
IL-1R-1; IL-1RT1; IL-1R-alpha; CD121a; IL-1 RI; IL1R; IL1RI; IL-1RI; p80; D2S1473; IL1RA |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu18-Thr332 |
Molecular Weight |
The protein has a predicted MW of 37 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-1R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 0.13ug/ml determined by ELISA (QC Test). The affinity constant of 0.51 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human IL-1R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-1 alpha, No tag with an affinity constant of 0.51 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human IL-1R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 0.13ug/ml determined by ELISA (QC Test). |
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Human IL-1R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human IL-1R1 is greater than 95% as determined by SEC-HPLC. |
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