Human IL-1R2/IL-1 RII/CD121b Protein (IL1-HM0R2)

  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-1R2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 0.21ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe14-Glu343

Catalog: IL1-HM0R2

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Product Description Recombinant Human IL-1R2/IL-1 RII/CD121b Protein is expressed from HEK293 without tag.It contains Phe14-Glu343.

Product Background

Interleukin (IL)-1 has been reported to be involved in the development of tuberculosis (TB). IL1R1 and IL1R2 encode a cytokine receptor that belongs to the IL-1 receptor family.IL1R2 (Interleukin 1 Receptor Type 2) is a Protein Coding gene. Diseases associated with IL1R2 include Endometriosis and Mastitis. Among its related pathways are Hematopoietic cell lineage and IL-1 signaling pathway. Gene Ontology (GO) annotations related to this gene include interleukin-1 receptor activity and interleukin-1, type II, blocking receptor activity.

Product Category

Recombinant Protein

Target

IL-1R2/IL-1 RII/CD121b
Accession

Species

Human

Exact Sequence

Phe14-Glu343
Molecular Weight The protein has a predicted MW of 37.78 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-1R2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 0.21ug/ml determined by ELISA. See testing image for detail.

Product Tag

No Tag

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-1R2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 0.21ug/ml determined by ELISA.
The purity of Human IL-1R2 is greater than 95% as determined by SEC-HPLC.
Human IL-1R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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