• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human IL-1 Beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 0.88 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Phe14-Glu343
Catalog #IL1-HM2R2
Price
Regular price $24750
Regular price Sale price $24750
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Human IL-1R2/IL-1 RII/CD121b Protein (IL1-HM2R2) Summary

Product Description

 Recombinant Human IL-1R2/IL-1 RII/CD121b Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Phe14-Glu343.

Product Background

 The IL-1 type II receptor (decoy RII) is a nonsignaling molecule the only established function of which is to capture IL-1 and prevent it from interacting with signaling receptor. The decoy RII is released in a regulated way from the cell surface.

Human IL-1R2/IL-1 RII/CD121b Protein (IL1-HM2R2) Specifications/Details

Protein
 IL-1R2/IL-1 RII/CD121b
Synonyms
 IL-1R-2; IL-1RT-2; IL-1RT2; CDw121b; IL-1R-beta; CD121b; IL1RB; IL1R2; IL-1 RII; MGC47725; IL-1R-β;IL1RT2; IL1R-beta; IL1RII; IL1R-β;IL-1R-b; IL1R-b
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Phe14-Glu343
Molecular Weight
 The protein has a predicted MW of 64.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-1 Beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 0.88 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-1R2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1 Beta, His Tag with an affinity constant of 0.88 nM as determined in SPR assay (Biacore T200). Human IL-1R2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1 Beta, His Tag with an affinity constant of 0.88 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-1 Beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). Immobilized Human IL-1 Beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
Human IL-1R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-1R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...