Human IL-1R2/IL-1 RII/CD121b Protein (IL1-HM2R2)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human IL-1 Beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.22μg/ml determined by ELISA. The affinity constant of 0.88 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Phe14-Glu343

Catalog: IL1-HM2R2

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Product Description Recombinant Human IL-1R2/IL-1 RII/CD121b Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Phe14-Glu343.
Product Category Recombinant Protein
Target IL-1R2/IL-1 RII/CD121b
Synonyms IL-1R-2; IL-1RT-2; IL-1RT2; CDw121b; IL-1R-beta; CD121b; IL1RB; IL1R2; IL-1 RII; MGC47725
Gene/Protein ID NP_004624.1
Species Human
Exact Sequence Phe14-Glu343
Molecular Weight The protein has a predicted MW of 64.4 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-1 Beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.22μg/ml determined by ELISA. The affinity constant of 0.88 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-1R2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1 Beta, His Tag with an affinity constant of 0.88 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-1 Beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.22μg/ml determined by ELISA.
Human IL-1R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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