Human IL-1R3/IL-1 RAcP Protein (ILR-HM2R3)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-1R3, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 19.2ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser21-Glu359

Catalog: ILR-HM2R3

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Product Description	Recombinant Human IL-1R3/IL-1 RAcP Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser21-Glu359.
Product Background	IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins. It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36. IL-1 R3 is a coreceptor for IL1RL2 in the IL-36 signaling system. IL-1 R3 is a coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.
Product Category	Recombinant Protein

Target	IL-1R3/IL-1 RAcP
Synonyms	IL1RAP; C3orf13; IL-1 R3; IL-1 RAcP
Gene/Protein ID	Q9NPH3-1
Species	Human
Exact Sequence	Ser21-Glu359
Molecular Weight	The protein has a predicted MW of 65.8 kDa. Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-1R3, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 19.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human IL-1R3, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-1R3 Antibody, hFc Tag with the EC50 of 19.2ng/ml determined by ELISA.
	The purity of Human IL-1R3 is greater than 95% as determined by SEC-HPLC.
	Human IL-1R3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.