• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-1RL1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-1RL1 Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA. The affinity constant of 17.57 pM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Lys19-Ser328
Catalog #ST2-HM1L2
Price
Regular price $22500
Regular price Sale price $22500
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Size
Avantor VWR Fisher Scientific

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Human IL-1RL1/sST2 Protein (ST2-HM1L2) Summary

Product Description

 Recombinant Human IL-1RL1/sST2 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Lys19-Ser328.

Product Background

 IL1RL1(also known as ST2)-is a member of the IL1-receptor family-and serves as the receptor for IL-33. The-ST2 protein-has two isoforms including sST2 and the membrane-bound ST2 receptor, referred to as ST2L. Preclinical studies have demonstrated that binding of IL-33 to ST2L elicits a cardioprotective effect. ST2/IL-33 signaling may play an important role in intestinal disease.

Human IL-1RL1/sST2 Protein (ST2-HM1L2) Specifications/Details

Protein
 IL-1RL1/ST2
Synonyms
 IL1RL1; DER4; ST2; T1; FIT-1; IL33R; ST2L; ST2V; IL-1 R4; IL1R4; Ly84; MGC32623;FIT1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Lys19-Ser328
Molecular Weight
 The protein has a predicted MW of 36.60 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-1RL1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-1RL1 Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA. The affinity constant of 17.57 pM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-33, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1RL1, His Tag with an affinity constant of 17.57 pM as determined in SPR assay (Biacore T200). Human IL-33, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1RL1, His Tag with an affinity constant of 17.57 pM as determined in SPR assay (Biacore T200).
Immobilized Human IL-1RL1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-1RL1 Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA. Immobilized Human IL-1RL1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-1RL1 Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA.
Immobilized Human IL-1RL1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-33, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA. Immobilized Human IL-1RL1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-33, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA.
Human IL-1RL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-1RL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-1RL1 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-1RL1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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