Name: Human IL-2 Protein (IL2-HE001)
Brand: KactusBio
SKU: IL2-HE001-100UG
Price: 132.0 USD
Availability: InStock

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 16.82 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ala21-Thr153

Catalog #IL2-HE001

Size

100UG

500UG

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Human IL-2 Protein (IL2-HE001) Summary

Product Description	Recombinant Human IL-2 Protein is expressed from E.coli without tag.It contains Ala21-Thr153.
Product Background	Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human IL-2 Protein (IL2-HE001) Specifications/Details

Protein	IL-2
Synonyms	IL2; T-cell growth factor; TCGF; Aldesleukin
Accession	P60568
Species	Human
Biotinylated	no
Amino Acid Range	Ala21-Thr153
Molecular Weight	The protein has a predicted 15.5 kDa same as Bis-Tris PAGE result.
Activity	The affinity constant of 16.82 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 100mM Acetic Acid. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 100mM Acetic Acid.). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-2 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 16.82 nM as determined in SPR assay (Biacore T200).
	Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-2, No Tag with an affinity constant of 0.25uM as determined in SPR assay (Biacore T200).
	Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200).
	Human IL-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.5-2 ng/mL (QC Test).
	The purity of Human IL-2 is greater than 95% as determined by SEC-HPLC.