Regular price
$270.00
Order by 4pm ET for delivery on:
Human IL-2 R alpha/CD25 Protein (CD5-HM125) Summary
Product Description |
Recombinant Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Glu22-Cys213. |
Product Background |
Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human IL-2 R alpha/CD25 Protein (CD5-HM125) Specifications/Details
Protein |
IL-2 R alpha/CD25 |
Synonyms |
CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu22-Cys213 |
Molecular Weight |
The protein has a predicted MW of 23.35 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His-Avi Tag (Cat. IL2-HM401B) with the EC50 of 93.3ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human IL-2 R alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2, His-Avi Tag (Cat. IL2-HM401B) with the EC50 of 93.3ng/ml determined by ELISA. |
|
|
Human IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Loaded Biotinylated Human IL-2, His-Avi Tag (Cat. IL2-HM401B) on Streptavidin-Biosensor can bind Human IL-2 R alpha, His Tag with an affinity constant of 35.70 nM as determined in BLI assay . |
|
|
The purity of Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
