• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-2, No Tag (Cat. IL2-HM001) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R alpha, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
  • Amino Acid Range: Glu22-Cys213
Catalog #CD5-HM225
Price
Regular price $27000
Regular price Sale price $27000
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Size
Avantor VWR Fisher Scientific

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Human IL-2 R alpha/CD25 Protein (CD5-HM225) Summary

Product Description

 Recombinant Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Glu22-Cys213.

Product Background

 Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Human IL-2 R alpha/CD25 Protein (CD5-HM225) Specifications/Details

Protein
 IL-2 R alpha/CD25
Synonyms
 CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR;IL2R alpha
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu22-Cys213
Molecular Weight
 The protein has a predicted MW of 47.68 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-2, No Tag (Cat. IL2-HM001) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R alpha, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-2, No Tag (Cat. IL2-HM001) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R alpha, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. Immobilized Human IL-2, No Tag (Cat. IL2-HM001) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R alpha, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Immobilized Human IL-2 R alpha, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. Immobilized Human IL-2 R alpha, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA.
Human IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human IL-2 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-2, No Tag (Cat. IL2-HM001) with an affinity constant of 10.10 nM as determined in BLI assay. Loaded Human IL-2 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-2, No Tag (Cat. IL2-HM001) with an affinity constant of 10.10 nM as determined in BLI assay.
The purity of Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC. The purity of Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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