• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-2, No Tag (Cat. IL2-HM001) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R alpha, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
  • Amino Acid Range: Glu22-Cys213
Catalog #CD5-HM225
Price
Regular price $27000
Regular price Sale price $27000
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Size
Avantor VWR Fisher Scientific

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Human IL-2 R alpha/CD25 Protein (CD5-HM225) Summary

Product Description

 Recombinant Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Glu22-Cys213.

Product Background

 Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

Human IL-2 R alpha/CD25 Protein (CD5-HM225) Specifications/Details

Protein
 IL-2 R alpha/CD25
Synonyms
 CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR;IL2R alpha
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu22-Cys213
Molecular Weight
 The protein has a predicted MW of 47.68 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-2, No Tag (Cat. IL2-HM001) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R alpha, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-2, No Tag (Cat. IL2-HM001) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R alpha, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. Immobilized Human IL-2, No Tag (Cat. IL2-HM001) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R alpha, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
Immobilized Human IL-2 R alpha, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. Immobilized Human IL-2 R alpha, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA.
Human IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-2 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC. The purity of Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...