Human IL-2 R beta/CD122 Protein (CD1-HM222)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-2 R beta, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, His Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test). The affinity constant of 2.88 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala27-Asp239

Catalog: CD1-HM222

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Product Description Recombinant Human IL-2 R beta/CD122 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala27-Asp239.

Product Background

IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains.

Product Category

Recombinant Protein

Target

IL-2 R beta/CD122
Accession

Species

Human

Amino Acid Range

Ala27-Asp239
Molecular Weight The protein has a predicted MW of 51.35 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-2 R beta, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, His Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test). The affinity constant of 2.88 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-2 R beta, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15, No Tag with an affinity constant of 2.88 nM as determined in SPR assay (Biacore T200).
Human IL-2 R beta, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.216 uM as determined in SPR assay (Biacore T200).
Immobilized Human IL-2 R beta, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, His Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test).
The purity of Human IL-2 R beta is greater than 95% as determined by SEC-HPLC.
Human IL-2 R beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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