Human IL-2 R beta/CD122 Protein (CD1-HM222)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-2 R beta, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, His Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test). The affinity constant of 2.88 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala27-Asp239
Catalog #CD1-HM222
Regular price $315.00
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Product Description
Recombinant Human IL-2 R beta/CD122 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala27-Asp239.
Product Background
IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains.
Product Category
Recombinant Protein
Target
IL-2 R beta/CD122
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ala27-Asp239
Molecular Weight
The protein has a predicted MW of 51.35 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IL-2 R beta, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, His Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test). The affinity constant of 2.88 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-2 R beta, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15, No Tag with an affinity constant of 2.88 nM as determined in SPR assay (Biacore T200).
Human IL-2 R beta, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.216 uM as determined in SPR assay (Biacore T200).
Immobilized Human IL-2 R beta, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-15Ra&IL-15, His Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test).
Human IL-2 R beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-2 R beta is greater than 95% as determined by SEC-HPLC.

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