Human IL-2 R beta&IL-2 R gamma Protein (ILR-HM2BG)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose 21.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala27-Thr240(IL-2 R beta) & Leu23-Ala262(IL-2 R gamma)
Catalog #ILR-HM2BG
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Product Description
 Recombinant Human IL-2 R beta&IL-2 R gamma Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala27-Thr240 (IL-2 R beta)&Leu23-Ala262 (IL-2 R gamma).
Product Background
 Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change.
Product Category
 Recombinant Protein
Target
 IL-2 R beta&IL-2 R gamma
Synonyms
 IL-2RB&IL-2RG; IL-2RG; IL-2R subunit gamma; IL-2R subunit beta; IL-2RB; IL15RB; IL-2 R beta&IL-2 R gamma
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala27-Thr240(IL-2 R beta) & Leu23-Ala262(IL-2 R gamma)
Molecular Weight
 The protein has a predicted MW of 51.3 kDa (IL-2 R beta)&54.6 kDa (IL-2 R gamma). Due to glycosylation, the protein migrates to 65-70 kDa&80-100 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose 21.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200).
Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose 21.5ng/ml determined by ELISA (QC Test).
Human IL-2 R beta&IL-2 R gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-2 R beta&IL-2 R gamma is greater than 95% as determined by SEC-HPLC.
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