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Human IL-2 R beta&IL-2 R gamma Protein (ILR-HM2BG) Summary
Product Description |
Recombinant Human IL-2 R beta&IL-2 R gamma Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus of IL-2 R beta and IL-2 R gamma.It contains Ala27-Thr240 (IL-2 R beta) and Leu23-Ala262 (IL-2 R gamma). |
Product Background |
Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human IL-2 R beta&IL-2 R gamma Protein (ILR-HM2BG) Specifications/Details
Protein |
IL-2 R beta&IL-2 R gamma |
Synonyms |
IL-2RB&IL-2RG; IL-2RG; IL-2R subunit gamma; IL-2R subunit beta; IL-2RB; IL15RB; IL-2 R beta&IL-2 R gamma; IL-2R subunit β; IL-2 R β&IL-2 R gamma; IL-2Rγ; IL-2R γ; IL-2 Rγ; IL-2 R γ |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala27-Thr240(IL-2 R beta) & Leu23-Ala262(IL-2 R gamma) |
Molecular Weight |
The protein has a predicted MW of 51.3 kDa (IL-2 R beta) and 54.6 kDa (IL-2 R gamma). Due to glycosylation, the protein migrates to 65-70 kDa and 80-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 90% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). |
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Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human IL-15RA&IL-15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 21.5ng/ml determined by ELISA (QC Test). |
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Human IL-2 R beta&IL-2 R gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. |
Other Available Proteins:
Finding available proteins...
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