Human IL-2 R beta&IL-2 R gamma Protein (ILR-HM2BG)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-15RA&IL-15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA. The affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala27-Thr240(IL-2 R beta) & Leu23-Ala262(IL-2 R gamma)

Catalog: ILR-HM2BG

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Product Description Recombinant Human IL-2 R beta&IL-2 R gamma Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Ala27-Thr240 (IL-2 R beta)&Leu23-Ala262 (IL-2 R gamma).
Product Category Recombinant Protein
Target IL-2 R beta&IL-2 R gamma
Synonyms IL-2RB&IL-2RG; IL-2RG; IL-2R subunit gamma; IL-2R subunit beta; IL-2RB; IL15RB; IL-2 R beta&IL-2 R gamma
Gene/Protein ID P14784(IL-2 R beta)&P31785-1(IL-2 R gamma)
Species Human
Exact Sequence Ala27-Thr240(IL-2 R beta) & Leu23-Ala262(IL-2 R gamma)
Molecular Weight The protein has a predicted MW of 51.3 kDa (IL-2 R beta)&54.6 kDa (IL-2 R gamma). Due to glycosylation, the protein migrates to 65-70 kDa&80-100 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-15RA&IL-15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA. The affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200).
Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-15RA&IL-15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 0.11μg/ml determined by ELISA.
The purity of Human IL-2 R beta&IL-2 R gamma is greater than 95% as determined by SEC-HPLC.
Human IL-2 R beta&IL-2 R gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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