Name: Human IL-21 Protein (IL2-HE021)
Brand: KactusBio
SKU: IL2-HE021-100UG
Price: 165.0 USD
Availability: InStock

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln32-Ser162

Catalog #IL2-HE021

Size

100UG

500UG

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Product Description	Recombinant Human IL-21 Protein is expressed from E.coli without tag.It contains Gln32-Ser162.
Product Background	Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	IL-21
Synonyms	Interleukin-21; IL-21; Za11; CVID11
Accession	Q9HBE4-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln32-Ser162
Molecular Weight	The protein has a predicted MW of 15.4 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA.
	Human IL-21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50-for this effect is <8 ng/mL (QC Test).
	The purity of Human IL-21 is greater than 95% as determined by SEC-HPLC.