Product Description |
Recombinant Human IL-21 Protein is expressed from E.coli without tag.It contains Gln32-Ser162. |
Product Background |
Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases. |
Product Category |
Recombinant Protein |
Target |
IL-21 |
Synonyms |
Interleukin-21; IL-21; Za11; CVID11 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln32-Ser162 |
Molecular Weight |
The protein has a predicted MW of 15.4 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.5). |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA. |
|
|
Human IL-21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50-for this effect is 1-3 ng/mL (QC Test). |
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