Human IL-21 Protein (IL2-HE021)
Human IL-21 Protein (IL2-HE021)
Human IL-21 Protein (IL2-HE021)
Human IL-21 Protein (IL2-HE021)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln32-Ser162
Catalog #IL2-HE021
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Product Description
 Recombinant Human IL-21 Protein is expressed from E.coli without tag.It contains Gln32-Ser162.
Product Background
 Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.
Product Category
 Recombinant Protein
Target
 IL-21
Synonyms
 Interleukin-21; IL-21; Za11; CVID11
Accession
Species
 Human
Amino Acid Range
 Gln32-Ser162
Molecular Weight
 The protein has a predicted MW of 15.4 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.
Product Tag
 No Tag
Express System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.5).
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA.
Human IL-21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50-for this effect is 1-3 ng/mL (QC Test).
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