Human IL-21 Protein (IL2-HE021)
Catalog: IL2-HE021
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| Product Description | Recombinant Human IL-21 Protein is expressed from E.coli without tag.It contains Gln32-Ser162. |
| Product Category | Recombinant Protein |
| Target | IL-21 |
| Synonyms | Interleukin-21; IL-21; Za11; CVID11 |
| Gene/Protein ID | Q9HBE4-1 |
| Species | Human |
| Exact Sequence | Gln32-Ser162 |
| Molecular Weight | The protein has a predicted MW of 15.4 kDa same as Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-21, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 63.6ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | No Tag |
| Express System | E.coli |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in 20mM NaAc, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.5). |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human IL-21, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 63.6ng/ml determined by ELISA. |
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The purity of Human IL-21 is greater than 95% as determined by SEC-HPLC. |
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Human IL-21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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