Human IL-21 Protein (IL2-HE021)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln32-Ser162

Catalog: IL2-HE021

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Product Description Recombinant Human IL-21 Protein is expressed from E.coli without tag.It contains Gln32-Ser162.

Product Background

Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.

Product Category

Recombinant Protein

Target

IL-21
Synonyms Interleukin-21; IL-21; Za11; CVID11

Gene/Protein ID

Q9HBE4-1

Species

Human

Exact Sequence

Gln32-Ser162
Molecular Weight The protein has a predicted MW of 15.4 kDa same as Tris-Bis PAGE result.

Activity

Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

No Tag

Express System

E.coli
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.5).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA.
Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50-for this effect is 1-3 ng/mL (QC Test).
Human IL-21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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