Human IL-21 Protein (IL2-HE021)

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln32-Ser162

Catalog: IL2-HE021

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Product Description	Recombinant Human IL-21 Protein is expressed from E.coli without tag.It contains Gln32-Ser162.
Product Background	Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.
Product Category	Recombinant Protein

Target	IL-21
Synonyms	Interleukin-21; IL-21; Za11; CVID11
Gene/Protein ID	Q9HBE4-1
Species	Human
Exact Sequence	Gln32-Ser162
Molecular Weight	The protein has a predicted MW of 15.4 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.5).
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human IL-21, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, hFc Tag with the EC50 of 42.3ng/ml determined by ELISA.
	Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50-for this effect is < 8 ng/mL.
	Human IL-21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.