Product Description |
Recombinant Human IL-21R Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys20-Glu232. |
Product Background |
IL-21 and IL-21R were highly expressed in the lesional skin and peripheral blood of psoriasis patients. IL-21 promoted CD4 T cells proliferation and Th17 cells differentiation and inhibiting Treg cells differentiation by upregulating RORγt expression and downregulating Foxp3 expression, with increased expression and secretion of IL-17A and IL-22.Microbial translocation and the associated immune activation during HIV-1 infection may lead to high expression levels of the IL-21R activation marker in RM B cells, a feature associated with increased apoptosis and a reduced number of these cells in the circulation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
IL-21R |
Synonyms |
IL-21 receptor; IL21R; CD360; NILR |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Cys20-Glu232 |
Molecular Weight |
The protein has a predicted MW of 25.65 kDa. Due to glycosylation, the protein migrates to 43-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-21 at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, His Tag with the EC50 of 34.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human IL-21 at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-21R, His Tag with the EC50 of 34.2ng/ml determined by ELISA. |
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Human IL-21R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human IL-21R is greater than 95% as determined by SEC-HPLC. |
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