Human IL-23 alpha&IL-12 beta Protein (IL2-HM1AB)
Human IL-23 alpha&IL-12 beta Protein (IL2-HM1AB)
Human IL-23 alpha&IL-12 beta Protein (IL2-HM1AB)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human IL-23R, hFc Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 46.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Arg20-Pro189 (IL-23 alpha) & Ile23-Ser328 (IL-12 beta)
Catalog #IL2-HM1AB
Regular price $346.50
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Product Description

Recombinant Human IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag at the C-terminus.It contains Arg20-Pro189 (IL-23 alpha)&Ile23-Ser328 (IL-12 beta).

Product Background

The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity.

Product Category

Recombinant Protein / Antibody discovery

Protein

IL-23 alpha&IL-12 beta

Synonyms

IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Arg20-Pro189 (IL-23 alpha) & Ile23-Ser328 (IL-12 beta)

Molecular Weight

The protein has a predicted MW of 19.77 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Due to glycosylation, the protein migrates to 23 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) based on Bis-Tris PAGE result.

Activity

Immobilized Human IL-23R, hFc Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 46.4ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human IL-23R, hFc Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 46.4ng/ml determined by ELISA (QC Test). Immobilized Human IL-23R, hFc Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 46.4ng/ml determined by ELISA (QC Test).
Immobilized Human IL-23 alpha&IL-12 beta, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 3.3 ng/ml determined by ELISA. Immobilized Human IL-23 alpha&IL-12 beta, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-IL-23 Antibody, hFc Tag with the EC50 of 3.3 ng/ml determined by ELISA.
Human IL-23 alpha&IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-23 alpha&IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC. The purity of Human IL-23 alpha&IL-12 beta is greater than 95% as determined by SEC-HPLC.

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