- Express System: HEK293
- Product Tag: C-His
- Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
- Activity: Immobilized Human IL-23R, hFc Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 46.4ng/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Arg20-Pro189 (IL-23 alpha) & Ile23-Ser328 (IL-12 beta)
Product Description |
Recombinant Human IL-23 alpha&IL-12 beta Protein is expressed from HEK293 with His tag at the C-terminus.It contains Arg20-Pro189 (IL-23 alpha)&Ile23-Ser328 (IL-12 beta). |
Product Background |
The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
IL-23 alpha&IL-12 beta |
Synonyms |
IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg20-Pro189 (IL-23 alpha) & Ile23-Ser328 (IL-12 beta) |
Molecular Weight |
The protein has a predicted MW of 19.77 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Due to glycosylation, the protein migrates to 23 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-23R, hFc Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 46.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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