• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA.
  • Amino Acid Range: Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta)
Catalog #ILB-HM4AB
Price
Regular price $52030
Regular price Sale price $52030
/
Size
Avantor VWR Fisher Scientific

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Non-biotinylated Human IL-23 alpha&Mouse IL-12 beta Protein (ILB-HM4AB) Summary

Product Description

 Recombinant Human IL-23 alpha&Mouse IL-12 beta Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of IL-23 alpha.It contains Arg20-Pro189(Human IL-23 alpha) and Met23-Ser335(Mouse IL-12 beta).

Product Background

 Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.

Non-biotinylated Human IL-23 alpha&Mouse IL-12 beta Protein (ILB-HM4AB) Specifications/Details

Protein
 IL-23 alpha&IL-12 beta
Synonyms
 IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β;IL12 b; IL12b; IL23 alpha&IL12 b; IL23 alpha&IL12b
Accession
Species
 Human&Mouse
Biotinylated
 no
Amino Acid Range
 Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta)
Molecular Weight
 The protein has a predicted MW of 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta). Due to glycosylation, the protein migrates to 22 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta) based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA. Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA.
Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Risankizumab, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA. Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Risankizumab, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
Human IL-23 alpha&Mouse IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-23 alpha&Mouse IL-12 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-23 alpha&Mouse IL-12 beta is greater than 90% as determined by SEC-HPLC. The purity of Human IL-23 alpha&Mouse IL-12 beta is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...