Human IL-23 alpha&Mouse IL-12 beta Protein (ILB-HM4AB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc with the EC50 of 0.36μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta)

Catalog: ILB-HM4AB

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Product Description Recombinant Human IL-23 alpha&Mouse IL-12 beta Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta).
Product Category Recombinant Protein
Target IL-23 alpha&IL-12 beta
Synonyms IL23 alpha; IL12 beta; IL23 alpha&IL12 beta
Gene/Protein ID Q9NPF7(Human IL-23 alpha)&P43432-1(Mouse IL-12 beta)
Species Human&Mouse
Exact Sequence Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta)
Molecular Weight The protein has a predicted MW of 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta). Due to glycosylation, the protein migrates to 22 kDa (Human IL-23 alpha) & 50-60 kDa (Mouse IL-12 beta) based on Tris-Bis PAGE result.
Activity Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc with the EC50 of 0.36μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc with the EC50 of 0.36μg/ml determined by ELISA.
The purity of Human IL-23 alpha&Mouse IL-12 beta is greater than 95% as determined by SEC-HPLC.
Human IL-23 alpha&Mouse IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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