Human IL-23 alpha&Mouse IL-12 beta Protein (ILB-HM4AB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta)

Catalog: ILB-HM4AB

$473.00
Size:

Order now get it on

Product Description Recombinant Human IL-23 alpha&Mouse IL-12 beta Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta).

Product Background

Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor.

Product Category

Recombinant Protein

Target

IL-23 alpha&IL-12 beta
Synonyms IL23 alpha; IL12 beta; IL23 alpha&IL12 beta

Gene/Protein ID

Q9NPF7(Human IL-23 alpha)&P43432-1(Mouse IL-12 beta)

Species

Human&Mouse

Exact Sequence

Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta)
Molecular Weight The protein has a predicted MW of 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta). Due to glycosylation, the protein migrates to 22 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta) based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.15ug/ml determined by ELISA.
The purity of Human IL-23 alpha&Mouse IL-12 beta is greater than 95% as determined by SEC-HPLC.
Human IL-23 alpha&Mouse IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in