Human IL-23 alpha&Mouse IL-12 beta Protein (ILB-HM4AB)
Catalog: ILB-HM4AB
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| Product Description | Recombinant Human IL-23 alpha&Mouse IL-12 beta Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta). |
| Product Category | Recombinant Protein |
| Target | IL-23 alpha&IL-12 beta |
| Synonyms | IL23 alpha; IL12 beta; IL23 alpha&IL12 beta |
| Gene/Protein ID | Q9NPF7(Human IL-23 alpha)&P43432-1(Mouse IL-12 beta) |
| Species | Human&Mouse |
| Exact Sequence | Arg20-Pro189(Human IL-23 alpha) & Met23-Ser335(Mouse IL-12 beta) |
| Molecular Weight | The protein has a predicted MW of 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta). Due to glycosylation, the protein migrates to 22 kDa (Human IL-23 alpha) & 50-60 kDa (Mouse IL-12 beta) based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.33μg/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.33μg/ml determined by ELISA. |
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The purity of Human IL-23 alpha&Mouse IL-12 beta is greater than 95% as determined by SEC-HPLC. |
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Human IL-23 alpha&Mouse IL-12 beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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