Human IL-23R Protein (ILR-HM323)

  • Express System: Expi293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-23 alpha&Mouse IL-12, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc with the EC50 of 0.36μg/ml determined by ELISA. The affinity constant of 11.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly24-Gly355

Catalog: ILR-HM323

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Product Description Recombinant Human IL-23R Protein is expressed from Expi293 with mFc at the C-terminal.It contains Gly24-Gly355.
Product Category Recombinant Protein
Target IL-23R
Synonyms IBD17; IL-23 R; IL-23 receptor
Gene/Protein ID Q5VWK5-1
Species Human
Exact Sequence Gly24-Gly355
Molecular Weight The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-23 alpha&Mouse IL-12, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc with the EC50 of 0.36μg/ml determined by ELISA. The affinity constant of 11.09 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-mFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-23R, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 11.09 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-23 alpha&Mouse IL-12, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc with the EC50 of 0.36μg/ml determined by ELISA.
The purity of Human IL-23R is greater than 95% as determined by SEC-HPLC.
Human IL-23R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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