Product Description |
Recombinant Human IL-23R Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Gly24-Gly355. |
Product Background |
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R).This receptor associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. |
Product Category |
Recombinant Protein |
Target |
IL-23R |
Synonyms |
IBD17; IL-23 R; IL-23 receptor |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly24-Gly355 |
Molecular Weight |
The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 36.4ng/ml determined by ELISA (QC Test). The affinity constant of 11.09 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-mFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human IL-23R, mFc Tag captured on CM5 Chip via Anti-Mouse Antibody can bind Human IL-23 alpha&IL-12 beta, His-Avi Tag with an affinity constant of 11.09 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Biotinylated Human IL-23 alpha&IL-12 beta, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 36.4ng/ml determined by ELISA (QC Test). |
|
|
Human IL-23R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human IL-23R is greater than 95% as determined by SEC-HPLC. |
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