Human IL-25/IL-17E Protein (IL2-HM125)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: The affinity constant of 0.81nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Tyr33-Gly177

Catalog: IL2-HM125

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Product Description Recombinant Human IL-25/IL-17E Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Tyr33-Gly177.
Product Category Recombinant Protein
Target IL-25/IL-17E
Synonyms IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25
Gene/Protein ID Q9H293-1
Species Human
Exact Sequence Tyr33-Gly177
Molecular Weight The protein has a predicted MW of 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.81nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25/IL-17E, His Tag with an affinity constant of 0.81nM as determined in SPR assay (Biacore T200).
The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC.
Human IL-25 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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