Human IL-25/IL-17E Protein (IL2-HM125)
Catalog: IL2-HM125
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| Product Description | Recombinant Human IL-25/IL-17E Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Tyr33-Gly177. |
| Product Category | Recombinant Protein |
| Target | IL-25/IL-17E |
| Synonyms | IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25 |
| Gene/Protein ID | Q9H293-1 |
| Species | Human |
| Exact Sequence | Tyr33-Gly177 |
| Molecular Weight | The protein has a predicted MW of 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 0.81nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25/IL-17E, His Tag with an affinity constant of 0.81nM as determined in SPR assay (Biacore T200). |
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The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC. |
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Human IL-25 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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