Human IL-25/IL-17E Protein (IL2-HM125)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-25, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 1.46ug/ml determined by ELISA (QC Test). The affinity constant of 0.81nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Tyr33-Gly177
Catalog #IL2-HM125
Regular price $315.00
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Product Description
Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr33-Gly177.
Product Background
IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.
Product Category
Recombinant Protein
Target
IL-25/IL-17E
Synonyms
IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Tyr33-Gly177
Molecular Weight
The protein has a predicted MW of 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IL-25, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 1.46ug/ml determined by ELISA (QC Test). The affinity constant of 0.81nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-25, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 1.46ug/ml determined by ELISA (QC Test).
Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC.

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