Product Description |
Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr33-Gly177. |
Product Background |
IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer. |
Product Category |
Recombinant Protein |
Target |
IL-25/IL-17E |
Synonyms |
IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Tyr33-Gly177 |
Molecular Weight |
The protein has a predicted MW of 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-25, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 1.46ug/ml determined by ELISA (QC Test). The affinity constant of 0.81nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human IL-25, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 1.46ug/ml determined by ELISA (QC Test). |
|
|
Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recently viewed
