Human IL-25/IL-17E Protein (IL2-HM125)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.81nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Tyr33-Gly177

Catalog: IL2-HM125

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Product Description Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr33-Gly177.

Product Background

IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.

Product Category

Recombinant Protein

Target

IL-25/IL-17E
Synonyms IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25
Accession

Species

Human

Amino Acid Range

Tyr33-Gly177
Molecular Weight The protein has a predicted MW of 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 0.81nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81nM as determined in SPR assay (Biacore T200).
The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC.
Human IL-25 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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