Human IL-25/IL-17E Protein (IL2-HM225)

Immobilized Human IL-17RB, His Tag (Cat.IL1-HM1RB) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-25, hFc Tag with the EC50 of 0.90ug/ml determined by ELISA.

Immobilized Human IL-25, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-25 Antibody, hFc Avi Tag with the EC50 of 11.1ng/ml determined by ELISA.

Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-17RB, His Tag (Cat.IL1-HM1RB) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-25, hFc Tag with the EC50 of 0.90ug/ml determined by ELISA.
Amino Acid Range: Tyr33-Gly177

Catalog #IL2-HM225

Price

$315⁰⁰

Size

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Human IL-25/IL-17E Protein (IL2-HM225) Summary

Product Description

Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Tyr33-Gly177.

Product Background

IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.

Human IL-25/IL-17E Protein (IL2-HM225) Specifications/Details

Protein	IL-25/IL-17E
Synonyms	IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25;IL25;Interleukin 25; IL 25
Accession	Q9H293-1
Species	Human
Biotinylated	no
Amino Acid Range	Tyr33-Gly177
Molecular Weight	The protein has a predicted MW of 42.68 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-17RB, His Tag (Cat.IL1-HM1RB) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-25, hFc Tag with the EC50 of 0.90ug/ml determined by ELISA.
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human IL-17RB, His Tag (Cat.IL1-HM1RB) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-25, hFc Tag with the EC50 of 0.90ug/ml determined by ELISA.
	Immobilized Human IL-25, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-25 Antibody, hFc Avi Tag with the EC50 of 11.1ng/ml determined by ELISA.
	Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC.