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Human IL-25/IL-17E Protein (IL2-HM225) Summary
Product Description |
Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Tyr33-Gly177. |
Product Background |
IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human IL-25/IL-17E Protein (IL2-HM225) Specifications/Details
Protein |
IL-25/IL-17E |
Synonyms |
IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Tyr33-Gly177 |
Molecular Weight |
The protein has a predicted MW of 42.68 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-17RB, His Tag (Cat.IL1-HM1RB) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-25, hFc Tag with the EC50 of 0.90ug/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human IL-17RB, His Tag (Cat.IL1-HM1RB) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-25, hFc Tag with the EC50 of 0.90ug/ml determined by ELISA. |
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Immobilized Human IL-25, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-25 Antibody, hFc Avi Tag with the EC50 of 11.1ng/ml determined by ELISA. |
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Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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