• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-17RB, His Tag (Cat.IL1-HM1RB) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-25, hFc Tag with the EC50 of 0.90ug/ml determined by ELISA.
  • Amino Acid Range: Tyr33-Gly177
Catalog #IL2-HM225
Price
Regular price $315.00
Regular price Sale price $315.00
/
Size
Avantor VWR Fisher Scientific

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Human IL-25/IL-17E Protein (IL2-HM225) Summary

Product Description

 Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Tyr33-Gly177.

Product Background

 IL-25, also known as IL-17E, is a member of the IL-17 cytokine family mostly produced by epithelial cells and innate immune cells. After binding to the IL-17RB/IL-17RA complex, IL-25 induces downstream signaling responses in epithelial cells and type 2 lymphocytes, which initiates, propagates, and sustains type 2 immunity. The function of IL-25 in allergic diseases such as asthma has been well established, and now also is extended to diseases such as inflammatory bowel disease and cancer.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human IL-25/IL-17E Protein (IL2-HM225) Specifications/Details

Protein
 IL-25/IL-17E
Synonyms
 IL-25; IL-17E; IL17E; Interleukin-17E; interleukin-25
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Tyr33-Gly177
Molecular Weight
 The protein has a predicted MW of 42.68 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-17RB, His Tag (Cat.IL1-HM1RB) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-25, hFc Tag with the EC50 of 0.90ug/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-17RB, His Tag (Cat.IL1-HM1RB) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-25, hFc Tag with the EC50 of 0.90ug/ml determined by ELISA. Immobilized Human IL-17RB, His Tag (Cat.IL1-HM1RB) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-25, hFc Tag with the EC50 of 0.90ug/ml determined by ELISA.
Immobilized Human IL-25, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-25 Antibody, hFc Avi Tag with the EC50 of 11.1ng/ml determined by ELISA. Immobilized Human IL-25, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-25 Antibody, hFc Avi Tag with the EC50 of 11.1ng/ml determined by ELISA.
Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-25 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-25 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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