||Arg21-Lys229(IL-27B) & Phe29-Pro243(IL-27A)
||The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 54-64 kDa based on Tris-Bis PAGE result.
||Immobilized Human IL-27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 16.8ng/ml determined by ELISA. The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail.
||> 95% as determined by Tris-Bis PAGE
||Less than 1EU per μg by the LAL method.