Human IL-3 Protein (IL3-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.276 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala20-Phe152
Catalog #IL3-HM201
Regular price $225.00
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Product Description

Recombinant Human IL-3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala20-Phe152.

Product Background

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Product Category

Recombinant Protein

Protein

IL-3

Synonyms

IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ala20-Phe152

Molecular Weight

The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

Activity

The affinity constant of 0.276 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-3 R alpha, His Tag with an affinity constant of 0.276 uM as determined in SPR assay (Biacore T200). Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-3 R alpha, His Tag with an affinity constant of 0.276 uM as determined in SPR assay (Biacore T200).
Human IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-3 is greater than 95% as determined by SEC-HPLC.

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