• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 4.13 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Thr17-Trp443
Catalog #IL3-HM1RB
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

Order by 4pm ET for delivery on:

Human IL-3 R Beta/CD131 Protein (IL3-HM1RB) Summary

Product Description

 Recombinant Human IL-3 R Beta/CD131 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Thr17-Trp443.

Product Background

 Interleukin-3 receptor (IL-3R) is a heterodimer that comprises an IL-3 specific alpha chain (IL-3R alpha) and a common beta chain (beta C) that is shared with the receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-5. The beta c subunit does not bind any cytokine by itself but is involved in the formation of high-affinity functional receptors for IL-5 and GM-CSF. These receptors belong to the cytokine receptor superfamily, but they are structurally and functionally more related to each other and thus make up a distinct subfamily.

Human IL-3 R Beta/CD131 Protein (IL3-HM1RB) Specifications/Details

Protein
 IL-3 R Beta/CD131
Synonyms
 CDw131; CD131; CSF2RB; IL3RB; IL5RB; AIC2A; Csfgmrb
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Thr17-Trp443
Molecular Weight
 The protein has a predicted MW of 50.30 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 4.13 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-3 R Beta, His Tag immobilized on CM5 Chip can bind Human GM-CSF, No Tag with an affinity constant of 4.13 uM as determined in SPR assay (Biacore T200) (QC Test). Human IL-3 R Beta, His Tag immobilized on CM5 Chip can bind Human GM-CSF, No Tag with an affinity constant of 4.13 uM as determined in SPR assay (Biacore T200) (QC Test).
Human IL-3 R Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-3 R Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-3 R Beta is greater than 95% as determined by SEC-HPLC. The purity of Human IL-3 R Beta is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...