Human IL-31 Protein (IL3-HM101)
Catalog: IL3-HM101
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Product Description | Recombinant Human IL-31 Protein is expressed from HEK293 with His tag at the N-terminal.It contains Ser24-Thr164. |
Product Category | Recombinant Protein |
Target | IL-31 |
Synonyms | Interleukin-31; IL-31;IL31 |
Gene/Protein ID | Q6EBC2-1 |
Species | Human |
Exact Sequence | Ser24-Thr164 |
Molecular Weight | The protein has a predicted MW of 16.9 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human IL-31, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.15μg/ml determined by ELISA (QC Test). The affinity constant of 1.5nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | N-His |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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