Human IL-31 Protein (IL3-HM101)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-31, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.15ug/ml determined by ELISA (QC Test). The affinity constant of 1.5nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ser24-Thr164

Catalog: IL3-HM101

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Product Description	Recombinant Human IL-31 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ser24-Thr164.
Product Background	Many autoimmune skin diseases, such as bullous pemphigoid (BP), psoriasis and certain types of chronic urticaria, are associated with intensive pruritus. While histamine and neuropeptides have previously been ascribed to play a role in itch that accompanies these diseases, recent evidence suggests that the pruritogenic cytokine interleukin (IL)-31 is a major driver of pruritic responses. IL-31 was originally shown to be produced by activated helper T cells, particularly Th2 cells, mast cells, macrophages and dendritic cells.
Product Category	Recombinant Protein

Target	IL-31
Synonyms	Interleukin-31； IL-31；IL31
Gene/Protein ID	Q6EBC2-1
Species	Human
Exact Sequence	Ser24-Thr164
Molecular Weight	The protein has a predicted MW of 16.9 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-31, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.15ug/ml determined by ELISA (QC Test). The affinity constant of 1.5nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-31 RA, hFc Tag captured on Protein A chip, can bind Human IL-31, His Tag with an affinity constant of 1.5nM as determined in a SPR assay (Biacore T200).
	Immobilized Human IL-31, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.15ug/ml determined by ELISA (QC Test).
	The purity of Human IL-31 is greater than 95% as determined by SEC-HPLC.
	Human IL-31 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.