Human IL-31 Protein (IL3-HM101)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-31, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.15μg/ml determined by ELISA (QC Test). The affinity constant of 1.5nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ser24-Thr164

Catalog: IL3-HM101

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Product Description Recombinant Human IL-31 Protein is expressed from HEK293 with His tag at the N-terminal.It contains Ser24-Thr164.
Product Category Recombinant Protein
Target IL-31
Synonyms Interleukin-31； IL-31；IL31
Gene/Protein ID Q6EBC2-1
Species Human
Exact Sequence Ser24-Thr164
Molecular Weight The protein has a predicted MW of 16.9 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-31, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.15μg/ml determined by ELISA (QC Test). The affinity constant of 1.5nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-31 RA, hFc Tag captured on Protein A chip, can bind Human IL-31, His Tag with an affinity constant of 1.5nM as determined in a SPR assay (Biacore T200).
Immobilized Human IL-31, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.15μg/ml determined by ELISA (QC Test).
The purity of Human IL-31 is greater than 95% as determined by SEC-HPLC.
Human IL-31 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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