Human IL-31 Protein (IL3-HM101)

  • Express System: Expi293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-31, His Tag at 5_g/ml (100_l/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.2_g/ml determined by ELISA. The affinity constant of 1.5nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ser24-Thr164

Catalog: IL3-HM101

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Product Description Recombinant Human IL-31 Protein is expressed from Expi293 with His tag at the N-terminal.It contains Ser24-Thr164.
Product Category Recombinant Protein
Target IL-31
Synonyms Interleukin-31_ IL-31_IL31
Gene/Protein ID Q6EBC2-1
Species Human
Exact Sequence Ser24-Thr164
Molecular Weight The protein has a predicted MW of 16.9 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human IL-31, His Tag at 5_g/ml (100_l/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.2_g/ml determined by ELISA. The affinity constant of 1.5nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-31 RA, hFc Tag captured on Protein A chip, can bind Human IL-31, His Tag with an affinity constant of 1.5nM as determined in a SPR assay (Biacore T200).
Immobilized Human IL-31, His Tag at 5_g/ml (100_l/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.2_g/ml determined by ELISA.
The purity of Human IL-31 is greater than 95% as determined by SEC-HPLC.
Human IL-31 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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