Human IL-31 Protein (IL3-HM101)
Human IL-31 Protein (IL3-HM101)
Human IL-31 Protein (IL3-HM101)
Human IL-31 Protein (IL3-HM101)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-31, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.53ug/ml determined by ELISA (QC Test). The affinity constant of 1.97 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ser24-Thr164
Catalog #IL3-HM101
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Product Description
 Recombinant Human IL-31 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ser24-Thr164.
Product Background
 Many autoimmune skin diseases, such as bullous pemphigoid (BP), psoriasis and certain types of chronic urticaria, are associated with intensive pruritus. While histamine and neuropeptides have previously been ascribed to play a role in itch that accompanies these diseases, recent evidence suggests that the pruritogenic cytokine interleukin (IL)-31 is a major driver of pruritic responses. IL-31 was originally shown to be produced by activated helper T cells, particularly Th2 cells, mast cells, macrophages and dendritic cells.
Product Category
 Recombinant Protein
Target
 IL-31
Synonyms
 Interleukin-31； IL-31；IL31
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser24-Thr164
Molecular Weight
 The protein has a predicted MW of 16.9 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-31, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.53ug/ml determined by ELISA (QC Test). The affinity constant of 1.97 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 N-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-31 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-31, His Tag with an affinity constant of 1.97 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-31, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.53ug/ml determined by ELISA (QC Test).
Human IL-31 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-31 is greater than 95% as determined by SEC-HPLC.
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