Human IL-31 RA-Protein (ILR-HM1RA)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-31 RA, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-31 RA Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test). The affinity constant of 82.4nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala20-Glu519

Catalog: ILR-HM1RA

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Product Description	Recombinant Human IL-31 RA-Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala20-Glu519.
Product Background	Interleukin-31 receptor A (IL-31RA) is a newly identified type I cytokine receptor, that is related to gp130, the common receptor of the interleukin (IL) -6 family cytokines. IL-31RA forms a functional receptor complex for IL-31 together with the beta subunit of oncostatin M receptor (OSMRbeta). OSMRbeta is expressed in a subset of small-sized nociceptive neurons of adult dorsal root ganglia (DRGs).IL-31 and OSM may thus have redundant functions in the development of OSMRbeta-expressing neurons.
Product Category	Recombinant Protein

Target	IL-31 RA
Synonyms	IL-31RA; IL-31R-alpha; Gp130-like receptor; CRL3; GPL; ZcytoR17; GLM-R; CRL3Glmr; GLM-RMGC125346; PRO21384
Gene/Protein ID	Q8NI17-1
Species	Human
Exact Sequence	Ala20-Glu519
Molecular Weight	The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 90-116 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-31 RA, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-31 RA Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test). The affinity constant of 82.4nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-31, His Tag immobilized on CM5 Chip can bind Human IL-31 RA, His Tag with an affinity constant of 82.4nM as determined in a SPR assay (Biacore T200).
	Immobilized Human IL-31 RA, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-31 RA Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA (QC Test).
	The purity of Human IL-31 RA-is greater than 95% as determined by SEC-HPLC.
	Human IL-31 RA-on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.