- Express System: E.coli
- Product Tag: No Tag
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human IL-33, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-1RL1, His Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).
- Amino Acid Range: Ser112-Thr270
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Human IL-33 Protein (IL3-HE001) Summary
Product Description |
Recombinant Human IL-33 Protein is expressed from E.coli without tag.It contains Ser112-Thr270. |
Product Background |
Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, playing a role in inflammatory, infectious and autoimmune diseases and expressed in the cellular nucleus in several tissues. High levels of IL-33 are expressed in epithelial barrier tissues and endothelial barriers. ST2 is a receptor for IL-33, expressed selectively on a subset of Th2 cells, mediating some of their functions. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human IL-33 Protein (IL3-HE001) Specifications/Details
Protein |
IL-33 |
Synonyms |
C9orf26 Z; DVS27; IL1F11; NF-HEV; NFEHEV; IL33; DV27; C9ORF26; RP11-575C20.2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser112-Thr270 |
Molecular Weight |
The protein has a predicted MW of 18 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-33, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-1RL1, His Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). |
Product Tag |
No Tag |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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