Name: Human IL-33 Protein (IL3-HM133)
Brand: KactusBio
SKU: IL3-HM133-100UG
Price: 375.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human IL-1RL1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-33, His Tag with the EC50 of 1.93ug/ml determined by ELISA.
Amino Acid Range: His109-Thr270

Catalog #IL3-HM133

Size

100UG

500UG

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Human IL-33 Protein (IL3-HM133) Summary

Product Description	Recombinant Human IL-33 Protein is expressed from HEK293 with His tag at the N-terminus.It contains His109-Thr270.
Product Background	Interleukin-33 (IL-33) is a cytokine belonging to the IL-1 family, playing a role in inflammatory, infectious and autoimmune diseases and expressed in the cellular nucleus in several tissues. High levels of IL-33 are expressed in epithelial barrier tissues and endothelial barriers. ST2 is a receptor for IL-33, expressed selectively on a subset of Th2 cells, mediating some of their functions.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human IL-33 Protein (IL3-HM133) Specifications/Details

Protein	IL-33
Synonyms	C9orf26 Z; DVS27; IL1F11; NF-HEV; NFEHEV; IL33; DV27; C9ORF26; RP11-575C20.2
Accession	NP_254274.1
Species	Human
Biotinylated	no
Amino Acid Range	His109-Thr270
Molecular Weight	The protein has a predicted MW of 19.43 kDa. Due to glycosylation, the protein migrates to 26-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-1RL1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-33, His Tag with the EC50 of 1.93ug/ml determined by ELISA.
Product Tag	N-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human IL-1RL1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-33, His Tag with the EC50 of 1.93ug/ml determined by ELISA.
	Immobilized Human IL-33, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-33 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
	Human IL-33 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IL-33 is greater than 90% as determined by SEC-HPLC.

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