Human IL-37 Protein (IL7-HE037)
Human IL-37 Protein (IL7-HE037)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Lys27-Asp192
Catalog #IL7-HE037
Regular price $315.00
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Product Description
Recombinant Human IL-37 Protein is expressed from E.coli without tag.It contains Lys27-Asp192.
Product Background
IL-37 is a unique member of the IL-1 family of cytokines, which functions as a natural suppressor of inflammatory and immune responses. Immune and non-immune cells produce IL-37 precursor following pro-inflammatory stimuli. Following activating cleavage by caspase-1, mature IL-37 translocates to the nucleus, where it suppresses transcription of pro-inflammatory genes.
Product Category
Recombinant Protein
Target
IL-37
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Lys27-Asp192
Molecular Weight
The protein has a predicted MW of 18.55 kDa same as Bis-Tris PAGE result.
Activity
The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
No Tag
Express System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 20mM PBS, 2mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-18BP, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-37, No Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
Human IL-37 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-37 is greater than 95% as determined by SEC-HPLC.

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