Human IL-4 Protein (IL4-HM001)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-4, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: His25-Ser153
Catalog #IL4-HM001
Regular price $225.00
/
Size

Order now, ships by:

Product Description

Recombinant Human IL-4 Protein is expressed from HEK293 without tag.It contains His25-Ser153.

Product Background

Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.

Product Category

Recombinant Protein / Antibody discovery

Protein

IL-4

Synonyms

IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1

Accession

Species

Human

Biotinylated

no

Amino Acid Range

His25-Ser153

Molecular Weight

The protein has a predicted MW of 14.96 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human IL-4, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

No Tag

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human IL-4, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA. Immobilized Human IL-4, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 9.8ng/ml determined by ELISA.
Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.35 ng/mL Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.35 ng/mL
The purity of Human IL-4 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-4 is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed