Human IL-4 Protein (IL4-HM001)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-4, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: His25-Ser153
Catalog #IL4-HM001
Regular price $247.50
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Human IL-4 Protein (IL4-HM001) Summary

Product Description

Recombinant Human IL-4 Protein is expressed from HEK293 without tag.It contains His25-Ser153.

Product Background

Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human IL-4 Protein (IL4-HM001) Specifications/Details

Protein
IL-4
Synonyms
IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
His25-Ser153
Molecular Weight
The protein has a predicted MW of 14.96 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IL-4, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

No Tag
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human IL-4, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. Immobilized Human IL-4, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.35 ng/mL Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.35 ng/mL
The purity of Human IL-4 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-4 is greater than 95% as determined by SEC-HPLC.
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