Human IL-4 Protein (IL4-HM401)
Human IL-4 Protein (IL4-HM401)
Human IL-4 Protein (IL4-HM401)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human IL-4 at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.2 ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: His25-Ser153
Catalog #IL4-HM401
Regular price $315.00
/
Size

Order now, ships by:

Product Description

Recombinant Human IL-4 is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His25-Ser153.

Product Background

Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4/IL-13 family. Interleukin-4/IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, and this cytokine is a ligand for interleukin 4 receptor.

Product Category

Recombinant Protein / Antibody discovery

Protein

IL-4

Synonyms

IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1

Accession

Species

Human

Biotinylated

no

Amino Acid Range

His25-Ser153

Molecular Weight

The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human IL-4 at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.2 ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

>95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human IL-4 at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.2 ng/ml determined by ELISA (QC Test). Immobilized Human IL-4 at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.2 ng/ml determined by ELISA (QC Test).
Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is <0.2 ng/mL. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is <0.2 ng/mL.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed