Human IL-4 Protein (IL4-HM401)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human IL-4 at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: His25-Ser153

Catalog: IL4-HM401

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Product Description Recombinant Human IL-4 is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His25-Ser153.

Product Background

Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.-.

Product Category

Recombinant Protein

Target

IL-4
Synonyms IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1
Accession

Species

Human

Amino Acid Range

His25-Ser153
Molecular Weight The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-4 at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-4 at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is <0.2 ng/mL.
Human IL-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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