Human IL-4 Protein (IL4-HM401)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human IL-4 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: His25-Ser153

Catalog: IL4-HM401

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Product Description	Recombinant Human IL-4 is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His25-Ser153.
Product Background	Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.-.
Product Category	Recombinant Protein

Target	IL-4
Synonyms	IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1
Gene/Protein ID	P05112
Species	Human
Exact Sequence	His25-Ser153
Molecular Weight	The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-4 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human IL-4 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test).
	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is <0.2 ng/mL.
	Human IL-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.