- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: >95% as determined by Bis-Tris PAGE
- Activity: Immobilized Human IL-4 at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.2 ng/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: His25-Ser153
Product Description |
Recombinant Human IL-4 is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His25-Ser153. |
Product Background |
Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4/IL-13 family. Interleukin-4/IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, and this cytokine is a ligand for interleukin 4 receptor. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
IL-4 |
Synonyms |
IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
His25-Ser153 |
Molecular Weight |
The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-4 at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 4.2 ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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