Human IL-4 Protein, Ultra Low Endotoxin (IL4-HM201-UL)

Immobilized Human IL-4 R alpha, His Tag (Cat. ILA-HM14R) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.

Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-4 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-4 R alpha, His Tag (Cat. ILA-HM14R) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
Amino Acid Range: His25-Ser153

Catalog #IL4-HM201-UL

Price

$363⁰⁰

Size

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Human IL-4 Protein, Ultra Low Endotoxin (IL4-HM201-UL) Summary

Product Description

Recombinant Human IL-4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains His25-Ser153.

Product Background

Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4/IL-13 family. Interleukin-4/IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, and this cytokine is a ligand for interleukin 4 receptor.

Human IL-4 Protein, Ultra Low Endotoxin (IL4-HM201-UL) Specifications/Details

Protein	IL-4
Synonyms	IL4; IL-4; interleukin 4; MGC79402; pitrakinra; B cell growth factor 1; BCDF; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; BSF-1;Interleukin-4; IL 4
Accession	P05112-1
Species	Human
Biotinylated	no
Amino Acid Range	His25-Ser153
Molecular Weight	The protein has a predicted MW of 40.9 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-4 R alpha, His Tag (Cat. ILA-HM14R) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human IL-4 R alpha, His Tag (Cat. ILA-HM14R) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-4, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
	Human IL-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IL-4 is greater than 95% as determined by SEC-HPLC.