Human IL-4 R alpha/CD124 Protein (ILA-HM14R)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-4 R alpha, His Tag at 1ug/ml (100ul/Well). Dose response curve for Biotinylated Human IL-4, His Tag with the EC50 of 6.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly24-His232

Catalog: ILA-HM14R

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Product Description Recombinant Human IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-His232.

Product Background

Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family.The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells.

Product Category

Recombinant Protein

Target

IL-4 R alpha/CD124
Synonyms CD124; IL-4 R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124
Accession

Species

Human

Exact Sequence

Gly24-His232
Molecular Weight The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-4 R alpha, His Tag at 1ug/ml (100ul/Well). Dose response curve for Biotinylated Human IL-4, His Tag with the EC50 of 6.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-4 R alpha, His Tag at 1ug/ml (100ul/Well). Dose response curve for Biotinylated Human IL-4, His Tag with the EC50 of 6.1ng/ml determined by ELISA.
The purity of Human IL-4 R alpha is greater than 95% as determined by SEC-HPLC.
Human IL-4 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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