Human IL-4 R alpha/CD124 Protein (ILA-HM14R)
Human IL-4 R alpha/CD124 Protein (ILA-HM14R)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human IL-4 R alpha, His Tag at 1 ug/ml (100 ul/Well). Dose response curve for Biotinylated Human IL-4, His Tag with the EC50 of 6.1 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Met26-His232
Catalog #ILA-HM14R
Regular price $270.00
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Product Description
Recombinant Human IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Met26-His232.
Product Background
Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells.
Product Category
Recombinant Protein
Target
IL-4 R alpha/CD124
Synonyms
CD124; IL-4 R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Met26-His232
Molecular Weight
The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human IL-4 R alpha, His Tag at 1 ug/ml (100 ul/Well). Dose response curve for Biotinylated Human IL-4, His Tag with the EC50 of 6.1 ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-4 R alpha, His Tag at 1 ug/ml (100 ul/Well). Dose response curve for Biotinylated Human IL-4, His Tag with the EC50 of 6.1 ng/ml determined by ELISA.
Human IL-4 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-4 R alpha is greater than 95% as determined by SEC-HPLC.

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