Product Description |
Recombinant Human IL-4 R alpha/CD124 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Met26-His232. |
Product Background |
Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. |
Product Category |
Recombinant Protein |
Protein |
IL-4 R alpha/CD124 |
Synonyms |
CD124; IL-4 R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met26-His232 |
Molecular Weight |
The protein has a predicted MW of 50.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-4, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 5.5 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human IL-4, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human IL-4 R alpha, hFc Tag with the EC50 of 5.5 ng/ml determined by ELISA. |
|
|
Human IL-4 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human IL-4 R alpha is greater than 95% as determined by SEC-HPLC. |
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