• Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human IL-5, His Tag (Cat. IL5-HM101) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
  • Amino Acid Range: Asp21-Trp342
Catalog #IL5-HM22R
Price
Regular price $27000
Regular price Sale price $27000
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Size
Avantor VWR Fisher Scientific

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Human IL-5 R alpha/CD125 Protein (IL5-HM22R) Summary

Product Description

 Recombinant Human IL-5 R alpha/CD125 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.It contains Asp21-Trp342.

Product Background

 Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5.

Human IL-5 R alpha/CD125 Protein (IL5-HM22R) Specifications/Details

Protein
 IL-5 R alpha/CD125
Synonyms
 CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560;IL5R alpha; IL5R subunit alpha
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asp21-Trp342
Molecular Weight
 The protein has a predicted MW of 62.73 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-5, His Tag (Cat. IL5-HM101) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
Product Tag
 N-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-5, His Tag (Cat. IL5-HM101) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. Immobilized Human IL-5, His Tag (Cat. IL5-HM101) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
Human IL-5 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-5 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human IL-5 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-5, His Tag with an affinity constant of 14.80 nM as determined in BLI assay. Loaded Human IL-5 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-5, His Tag with an affinity constant of 14.80 nM as determined in BLI assay.
The purity of Human IL-5 R alpha is greater than 90% as determined by SEC-HPLC. The purity of Human IL-5 R alpha is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...