Human IL-5 R alpha/CD125 Protein (ILR-HM45R)
Human IL-5 R alpha/CD125 Protein (ILR-HM45R)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 5.70 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asp21-Glu335
Catalog #ILR-HM45R
Regular price $315.00
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Product Description

Recombinant Human IL-5 R alpha/CD125 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp21-Glu335.

Product Background

Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5.

Product Category

Recombinant Protein / Antibody discovery

Protein

IL-5 R alpha/CD125

Synonyms

CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; MGC26560

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Asp21-Glu335

Molecular Weight

The protein has a predicted MW of 38.7 kDa. Due to glycosylation, the protein migrates to 50-68 kDa based on Bis-Tris PAGE result.

Activity

The affinity constant of 5.70 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IL-5, His-Avi Tag immobilized on CM5 Chip can bind Human IL-5 R alpha, His-Avi Tag with an affinity constant of 5.70 nM as determined in SPR assay (Biacore T200). Human IL-5, His-Avi Tag immobilized on CM5 Chip can bind Human IL-5 R alpha, His-Avi Tag with an affinity constant of 5.70 nM as determined in SPR assay (Biacore T200).
Human IL-5R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-5R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-5R alpha is greater than 95% as determined by SEC-HPLC. The purity of Human IL-5R alpha is greater than 95% as determined by SEC-HPLC.

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