Human IL-6 Protein (IL6-HE001)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human IL-6 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 48.7ng/ml determined by ELISA. The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Val30-Met212

Catalog: IL6-HE001

$210.00
Size:

Order now get it on

Product Description Recombinant Human IL-6 is expressed from E.coli without tag.It contains Val30-Met212.

Product Background

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.

Product Category

Recombinant Protein

Target

IL-6
Synonyms IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF

Gene/Protein ID

P05231

Species

Human

Exact Sequence

Val30-Met212
Molecular Weight The protein has a predicted 20.8 kDa same as Tris-Bis PAGE result.

Activity

Immobilized Human IL-6 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 48.7ng/ml determined by ELISA. The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

No Tag

Express System

E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-6 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 48.7ng/ml determined by ELISA.
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.1 - 0.5 ng/mL (QC Test).
The purity of Human IL-6 is greater than 90% as determined by SEC-HPLC.
Human IL-6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in