Human IL-6 Protein (IL6-HE001)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Val30-Met212
Catalog #IL6-HE001
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Product Description
 Recombinant Human IL-6 is expressed from E.coli without tag.It contains Val30-Met212.
Product Background
 IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.
Product Category
 Recombinant Protein
Target
 IL-6
Synonyms
 IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Val30-Met212
Molecular Weight
 The protein has a predicted 20.8 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 No Tag
Express System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.05EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA (QC Test).
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.1 - 0.5 ng/mL (QC Test).
Human IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-6 is greater than 90% as determined by SEC-HPLC.
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