• Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA. The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Val30-Met212
Catalog #IL6-HE001
Price
Regular price $23100
Regular price Sale price $23100
/
Size
Avantor VWR Fisher Scientific

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Human IL-6 Protein (IL6-HE001) Summary

Product Description

 Recombinant Human IL-6 is expressed from E.coli without tag.It contains Val30-Met212.

Product Background

 IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.

Human IL-6 Protein (IL6-HE001) Specifications/Details

Protein
 IL-6
Synonyms
 IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF;IFN-β-2;BSF2; MGI2A;IFN-b-2; IFN-b2;Interleukin 6; IL 6
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Val30-Met212
Molecular Weight
 The protein has a predicted 20.8 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA. The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
Product Tag
 No Tag
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.05 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA. Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA.
Human IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.1 - 0.8 ng/mL. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.1 - 0.8 ng/mL.
Loaded Human IL-6 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-6, No Tag with an affinity constant of 16.50 nM as determined in BLI assay . Loaded Human IL-6 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-6, No Tag with an affinity constant of 16.50 nM as determined in BLI assay .
The purity of Human IL-6 is greater than 90% as determined by SEC-HPLC. The purity of Human IL-6 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...