Human IL-6 Protein (IL6-HE001)
Catalog: IL6-HE001
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Product Description | Recombinant Human IL-6 is expressed from E.coli without tag.It contains Val30-Met212. |
Product Category | Recombinant Protein |
Target | IL-6 |
Synonyms | IFN-beta-2; IL6; IL-6; BSF-2; CDF; MGI-2A; Interleukin-6; HSF; IFNB2; HGF |
Gene/Protein ID | P05231 |
Species | Human |
Exact Sequence | Val30-Met212 |
Molecular Weight | The protein has a predicted 20.8 kDa same as Tris-Bis PAGE result. |
Activity | Immobilized Human IL-6 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 32.6ng/ml determined by ELISA. The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | No Tag |
Express System | E.coli |
Purity | > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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