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Human IL-6 R alpha/CD126 Protein (ILR-HM26R) Summary
Product Description |
Recombinant Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu20-Pro365. |
Product Background |
Serum levels of interleukin-6 (IL-6) are increased in patients with type 2 diabetes (T2D). IL-6 exerts its pleiotropic effects via the IL-6 α-receptor (IL-6R), which exists in membrane-bound and soluble (sIL-6R) forms and activates cells via the β-receptor glycoprotein 130 (gp130). |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human IL-6 R alpha/CD126 Protein (ILR-HM26R) Specifications/Details
Protein |
IL-6 R alpha/CD126 |
Synonyms |
IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; IL6R; CD126; IL6RQ; gp80 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu20-Pro365 |
Molecular Weight |
The protein has a predicted MW of 65.3 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA (QC Test). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA (QC Test). |
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Immobilized Human IL-6 R alpha, hFc Tag at 2ug/ml (100ul/well) on the Human IL-6, No Tag precoated plate (5ug/ml). Dose response curve for Human gp130, His Tag with the EC50 of 49.6ng/ml determined by ELISA. |
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Human IL-6 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human IL-6 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-6, No Tag with an affinity constant of 16.50 nM as determined in BLI assay . |
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The purity of Human IL-6 R alpha is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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