Name: Non-biotinylated Human IL-6 R alpha/CD126 Protein (ILR-HM46R)
Brand: KactusBio
SKU: ILR-HM46R-100UG
Price: 346.5 USD
Availability: InStock

SPR sensorgram of Human IL-6 R Alpha (CD126) protein binding, showing response units (RU) over time for different analyte concentrations.

Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).

Immobilized Human IL-6, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test).

Human IL-6 R alpha on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

Loaded Human IL-6 R alpha, His Tag on Anti-His-Biosensor can bind Human IL-6, No Tag with an affinity constant of 30.80 nM as determined in BLI assay (Gator® Prime).

The purity of- Human IL-6 R alpha is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-6, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Leu20-Pro365

Catalog #ILR-HM46R

Size

100UG

500UG

Order now, ships by:

[{"id":42618369933534,"title":"100UG","option1":"100UG","option2":null,"option3":null,"sku":"ILR-HM46R-100UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Non-biotinylated Human IL-6 R alpha\/CD126 Protein (ILR-HM46R) - 100UG","public_title":"100UG","options":["100UG"],"price":34650,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":43584271974622,"title":"500UG","option1":"500UG","option2":null,"option3":null,"sku":"ILR-HM46R-500UG","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"Non-biotinylated Human IL-6 R alpha\/CD126 Protein (ILR-HM46R) - 500UG","public_title":"500UG","options":["500UG"],"price":115500,"weight":5,"compare_at_price":null,"inventory_management":"shopify","barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}]

Non-biotinylated Human IL-6 R alpha/CD126 Protein (ILR-HM46R) Summary

Product Description	Recombinant Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu20-Pro365.
Product Background	The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R alpha and gp130 have been cloned, sequenced, and expressed.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Non-biotinylated Human IL-6 R alpha/CD126 Protein (ILR-HM46R) Specifications/Details

Protein	IL-6 R alpha/CD126
Synonyms	IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; IL6R; CD126; IL6RQ; gp80
Accession	P08887-1
Species	Human
Biotinylated	no
Amino Acid Range	Leu20-Pro365
Molecular Weight	The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 65-78 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-6, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-6, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test).
	Human IL-6 R alpha on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	Loaded Human IL-6 R alpha, His Tag on Anti-His-Biosensor can bind Human IL-6, No Tag with an affinity constant of 30.80 nM as determined in BLI assay (Gator® Prime).
	The purity of- Human IL-6 R alpha is greater than 95% as determined by SEC-HPLC.