Human IL-6 R alpha/CD126 Protein (ILR-HM46R)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-6, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.15ug/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu20-Pro365
Catalog #ILR-HM46R
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Product Description
 Recombinant Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu20-Pro365.
Product Background
 The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R alpha and gp130 have been cloned, sequenced, and expressed.
Product Category
 Recombinant Protein
Target
 IL-6R alpha/CD126
Synonyms
 IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; IL6R; CD126; IL6RQ; gp80
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu20-Pro365
Molecular Weight
 The protein has a predicted MW of 41.4 kDa. Due to glycosylation, the protein migrates to 65-78 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-6, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.15ug/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-6, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.15ug/ml determined by ELISA (QC Test).
Human IL-6 R alpha on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of- Human IL-6 R alpha is greater than 95% as determined by SEC-HPLC.

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