Human IL-7 Protein (IL7-HE001)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-7 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 55.1ng/ml determined by ELISA. The affinity constant of 4.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Asp26-His177

Catalog: IL7-HE001

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Product Description Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177.
Product Category Recombinant Protein
Target IL-7
Synonyms IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF
Gene/Protein ID P13232
Species Human
Exact Sequence Asp26-His177
Molecular Weight The protein has a predicted MW of 17.5 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human IL-7 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 55.1ng/ml determined by ELISA. The affinity constant of 4.18 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag No Tag
Express System E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 25mM PB, 250mM NaCl (pH 6.8). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 25mM PB, 250mM NaCl (pH 6.8).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-7 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 4.18 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-7 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 55.1ng/ml determined by ELISA.
Measured in a cell proliferation assay using murine 2E8 cells. The ED50-for this effect is-0.1 - 0.5 ng/mL (QC tested).
The purity of Human IL-7 is greater than 95% as determined by SEC-HPLC.
Human IL-7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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