Human IL-7 Protein (IL7-HE001)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-7 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 22.66 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Asp26-His177

Catalog: IL7-HE001

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Product Description Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177.
Product Category Recombinant Protein
Target IL-7
Synonyms IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF
Gene/Protein ID P13232
Species Human
Exact Sequence Asp26-His177
Molecular Weight The protein has a predicted MW of 17.5 kDa same as Tris-Bis PAGE result.
Activity Immobilized Human IL-7 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 22.66 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag No Tag
Express System E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in 25mM PB, 250mM NaCl (pH 6.8). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Human IL-7R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 22.66 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-7 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA.
Human IL-7, No Tag stimulates proliferation of PHA-pretreated human peripheral blood mononuclear cell (PBMC) with the EC50 for this effect is 3.5ng/mL.
The purity of Human IL-7 is greater than 95% as determined by SEC-HPLC.
Human IL-7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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