Human IL-7 Protein (IL7-HE001)
Human IL-7 Protein (IL7-HE001)
Human IL-7 Protein (IL7-HE001)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA (QC tested). The affinity constant of 7.15 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Asp26-His177
Catalog #IL7-HE001
Regular price $231.00
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Human IL-7 Protein (IL7-HE001) Summary

Product Description

Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177.

Product Background

Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human IL-7 Protein (IL7-HE001) Specifications/Details

Protein
IL-7
Synonyms
IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Asp26-His177
Molecular Weight
The protein has a predicted MW of 17.5 kDa same as Bis-Tris PAGE result.
Activity
Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA (QC tested). The affinity constant of 7.15 nM as determined in SPR assay (Biacore T200).
Product Tag
No Tag
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-7 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 7.15 nM as determined in SPR assay (Biacore T200). Human IL-7 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 7.15 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA (QC tested). Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA (QC tested).
Human IL-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using murine 2E8 cells. The ED50-for this effect is-0.1 - 0.5 ng/mL (QC tested). Measured in a cell proliferation assay using murine 2E8 cells. The ED50-for this effect is-0.1 - 0.5 ng/mL (QC tested).
The purity of Human IL-7 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-7 is greater than 95% as determined by SEC-HPLC.
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