Product Description |
Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177. |
Product Background |
Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor. |
Product Category |
Recombinant Protein |
Target |
IL-7 |
Synonyms |
IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp26-His177 |
Molecular Weight |
The protein has a predicted MW of 17.5 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA (QC tested). The affinity constant of 7.15 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human IL-7 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 7.15 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA (QC tested). |
|
|
Measured in a cell proliferation assay using murine 2E8 cells. The ED50-for this effect is-0.1 - 0.5 ng/mL (QC tested). |
|
|
Human IL-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human IL-7 is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recently viewed
